HIV感染者T细胞NKG2C/NKG2A表达及与疾病进展关系研究  

Research on expression of NKG2C/NKG2A receptors on T cells in HIV infected individuals and relationship with disease progression

作　　者：陶安福 何磊[1] 张子宁[1] 傅雅静[1] 刘静[1] 胡清海[1] 姜拥军[1] TAO An-Fu HE Lei ZHANG Zi-Ning FU Ya-Jing LIU Jing HU Qing-Hai JIANG Yong-Jun.(Key Laboratpry of AIDS Immunology of Ministry of Health, Department of Laboratory Medicine, the First Hospital of China Medicql University, Shenyang 110001, China)

机构地区：[1]中国医科大学附属第一医院检验科卫生部艾滋病免疫学重点实验室,沈阳110001

出　　处：《中国免疫学杂志》2017年第1期90-93,98,共5页Chinese Journal of Immunology

基　　金：十二五科技重大专项(2014ZX10001002-001-004);基础临床紧密结合平台项目(医大发字[2013]5号)

摘　　要：目的:研究HIV慢性感染者和HARRT治疗者T细胞NKG2C/NKG2A受体的表达变化,探讨其与疾病进展的关系。方法:选取HIV慢性感染者、接受HAART治疗的HIV感染者以及健康人的外周血细胞,通过荧光抗体染色,利用流式细胞仪检测T细胞上表达的NKG2C/NKG2A受体。结果:HIV慢性感染者NKG2C^+T细胞,NKG2A^+T细胞和NKG2C^+NKG2A-T细胞百分比明显高于健康对照组(P=0.025、P=0.032、P=0.029),HARRT治疗组则明显低于HIV慢性感染者(P=0.033、P=0.037、P=0.018),恢复到正常水平,与健康对照组相比无统计学差异。HIV慢性感染者外周血CD4^+T淋巴细胞绝对数和表达NKG2A、NKG2C^+NKG2A^+和NKG2C-NKG2A^+的T细胞呈负相关(r=-0.697,P<0.000 1;r=-0.463,P=0.015;r=-0.693,P<0.000 1),HIV慢性感染者外周血T细胞上表达的NKG2C与NKG2A的比值与CD4^+T淋巴细胞绝对数呈正相关(r=0.476,P=0.012)。结论:HIV感染者T细胞表面NKG2C和NKG2A的表达研究具有重要意义,为HIV感染的临床预后评估提供科学依据。Objective： To study the changes of NKG2C/NKG2 A expressed on T cells in HIV chronically infected individuals and HAART-treatment AIDS patients and the relationship with disease progression of HIV. Methods： We collected peripheral blood from HIV chronically infected individuals,HAART-treatment AIDS patients and healthy human and used the flow cytometry by staining fluorescent antibody to detect the NKG2 C / NKG2 A receptors expressed on T cells. Results： NKG2C＋,NKG2A＋and NKG2C＋NKG2A-expressed on T cells in HIV chronically infected individuals were significantly higher than the healthy control group（ P =0. 025,P = 0. 032,P = 0. 029）,while in HAART-treatment AIDS patients were significantly lower than that in HIV chronically infected individuals（ P = 0. 033,P = 0. 037,P = 0. 018）,returned to the normal levels with no significant difference compared with the healthy control group. The absolute number of peripheral blood CD4＋T lymphocytes in HIV chronically infected individuals was negative correlation with T cells which expressing NKG2A＋,NKG2C＋NKG2A＋and NKG2C-NKG2A＋（ r =- 0. 697,P〈0. 000 1; r =- 0. 463,P= 0. 015; r =- 0. 693,P〈0. 000 1）. What was more,the absolute number of peripheral blood CD4＋T lymphocytes had positive correlation with the ratio of NKG2 C and NKG2 A expressed on T cells receptor in HIV chronically infected individuals（ r = 0. 476,P =0. 012）. Conclusion： Studying the expression of NKG2 C and NKG2 A receptors on T cell has great significance in HIV infected individuals,which may provide a scientific basis for clinical prognosis of HIV infection.

关 键 词：HIV感染者 T细胞 NKG2C NKG2A 

分 类 号：R512.91[医药卫生—内科学]