Irbesartan对冠状动脉粥样硬化小鼠的治疗作用及可能机制  

Therapeutical effect of Irbesartan for coronary atherosclerosis mice( ApoE^(-/-)) and its possible mechanisms

作　　者：余毅[1] 李增棋[1] 廖剑[1] YU Yi LI Zeng-Qi LIAO Jian.(Department of Cardiac Surgery, First Affiliated Hospital of Fujian Medical University, Fuzhou 350004, Chin)

机构地区：[1]福建医科大学附属第一医院心脏外科,福州350004

出　　处：《中国免疫学杂志》2017年第1期126-129,共4页Chinese Journal of Immunology

摘　　要：目的:明确Irbesartan对冠状动脉粥样硬化小鼠的治疗作用,并进一步分析可能的机制。方法:将16只雄性Apo E-/-小鼠给予高脂饮食(1.25%胆固醇,10%脂肪)构建冠心病模型。模型建立后将小鼠随机分为治疗组[Irbesartan,50mg/(kg·d),4周]及对照组(等量生理盐水灌胃对照)。采用HE、ELISA、Western blot及免疫荧光技术分析疗效及机制。结果:治疗组小鼠动脉粥样斑块面积显著低于对照组(P<0.05)。治疗组血管内IL-1β、IL-6及TNF-α水平显著低于对照组(P<0.05)。治疗组小鼠血管周围脂肪组织内PPAR-γ及Adiponectin水平显著升高,而Leptin水平显著降低,与对照组相比差异具有统计学意义(P<0.05)。Western blot及免疫荧光证实,Irbesartan显著抑制了治疗组小鼠血管周围脂肪组织内的NF-κB信号通路。结论:Irbesartan通过调节血管周围脂肪组织内PPAR-γ-NF-κb(p65)信号通路,调节血管周围脂肪组织功能及炎症,从而发挥抗动脉粥样硬化疗效。Objective： To analyze the therapeutical effect of Irbesartan for coronary atherosclerosis mice（ ApoE-/-） and its possible mechanisms. Methods： A total of 16 male Apo E-/-mice were randomly divided into control group and Treatment group[Irbesartan,50 mg/（ kg·d）,4 weeks]. HE,immunofluorescence,Western blot and ELISA were performed to analyze the effect of Irbesartan on ApoE-/-and the changes of related signaling pathways. Results： Compared with control group,the treatment group had lower atheroma macular areas and inflammatory cytokines in blood vessel（ P〈0. 05）. Treatment group had lower levels of leptin,but higher levels of PPAR-γ and adiponectin in perivascular adipose tissues（ PVAT） than these of control group,the difference were statistically significant（ P〈0. 05）. Western blot and immunofluorescence analysis shown that Irbesartan treatment significantly depressed the expression of p-p65 and p-IKK in PVAT when compared with these of control group（ P〈0. 05）. Conclusion： Irbesartan has significantly therapeutic effect on ApoE-/-mice,the possible mechanisms including anti-inflammatory effects in PVAT,improved the adipose tissue function and regulated the PPAR-γ-NF-κB signaling pathways.

关 键 词：厄贝沙坦 动物模型 动脉粥样硬化 脂肪组织 炎症 

分 类 号：R541.7[医药卫生—心血管疾病]