Chaetocin reactivates the lytic replication of Epstein-Barr virus from latency via reactive oxygen species  

作　　者：Shilun Zhang Juan Yin Jiang Zhong 

机构地区：[1]Department of Microbiology and Microbial Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China

出　　处：《Science China(Life Sciences)》2017年第1期66-71,共6页中国科学（生命科学英文版）

摘　　要：Oxidative stress, regarded as a negative effect of free radicals in vivo, takes place when organisms suffer from harmful stimuli. Some viruses can induce the release of reactive oxygen species （ROS） in infected cells, which may be closely related with their pathogenicity. In this report, chaetocin, a fimgal metabolite reported to have antimicrobial and cytostatic activity, was studied for its effect on the activation of latent Epstein-Barr virus （EBV） in B95-8 cells. We found that chaetocin remarkably up-regulated EBV lytic transcription and DNA replication at a low concentration （50 nmol L-l）. The activation of latent EBV was accompanied by an increased cellular ROS level. N-acetyl-L-cysteine （NAC）, an ROS inhibitor, suppressed chaetocin-induced EBV activation. Chaetocin had little effect on histone H3K9 methylation, while NAC also significantly reduced H3K9 methylation. These results suggested that chaetocin reactivates latent EBV primarily via ROS pathways.Oxidative stress,regarded as a negative effect of free radicals in vivo,takes place when organisms suffer from harmful stimuli.Some viruses can induce the release of reactive oxygen species(ROS) in infected cells,which may be closely related with their pathogenicity.In this report,chaetocin,a fungal metabolite reported to have antimicrobial and cytostatic activity,was studied for its effect on the activation of latent Epstein-Barr virus(EBV) in B95-8 cells.We found that chaetocin remarkably up-regulated EB V lytic transcription and DNA replication at a low concentration(50 nmol L^(-1).The activation of latent EBV was accompanied by an increased cellular ROS level.N-acetyl-L-cy steine(NAC),an ROS inhibitor,suppressed chaetocin-induced EBV activation.Chaetocin had little effect on histone H3K9 methylation,while NAC also significantly reduced H3K9 methylation.These results suggested that chaetocin reactivates latent EBV primarily via ROS pathways.

关 键 词：Epstein-Barr virus reactive oxygen species chaetocin 

分 类 号：R373[医药卫生—病原生物学]