不同细胞对DNA六螺旋纳米结构的摄取研究  

The uptake behavior of DNA six-helix nanostructure with different mammalian cell lines

作　　者：刘卓[1,2] 李凡[1,2] 郭琳洁[1] 赵彦[1,2] 李江[1] 刘小果[1] 王丽华[1] 樊春海[1] Zhuo Liu Fan Li Linjie Guo Yan Zhao Jiang Li Xiaoguo Llu Lihua Wang Chunhai Fan(Division of Physical Biology ＆ Bioimaging Center, Shanghai Synchrotron Radiation Facility, Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China University of Chinese Academy of Sciences, Beijing 100049, China)

机构地区：[1]中国科学院上海应用物理研究所物理生物学实验室,上海光源国家科学中心(筹)生物成像中心,上海201800 [2]中国科学院大学,北京100049

出　　处：《中国科学：化学》2017年第1期109-115,共7页SCIENTIA SINICA Chimica

基　　金：国家重点基础研究发展计划(编号:2013CB932803);国家自然科学基金(编号:31371015,11575278);上海市纳米专项(编号:13NM1402300);上海市科技人才计划(编号:14R21411500)资助项目

摘　　要：DNA纳米结构具有优异的生物相容性和高效的载药能力等性质,在生命科学和医学领域有很好的应用前景.然而,结构简单、分子量小的单链DNA(single strand DNA,ssDNA)进入细胞内的效率很低,且很快就被酶降解,无法完成载药任务.针对这种现状,本课题组通过DNA的自组装,将相互杂交的单链DNA构建为六螺旋(six-helix)结构,并对这种结构的稳定性和不同细胞的摄取情况进行了研究,发现与单链DNA相比,DNA六螺旋结构的稳定性更好,细胞摄取效率大幅提高,是一种潜在的药物载体.DNA nanostructures have great application potentials in biology and medical science, due to its excellent biocompatibility and drug loading ability. However, single strand DNA （ssDNA） with simple structures and small molecule weights are proved to be poor carriers because they cannot enter cells effectively and will be degraded by DNA enzyme rapidly. In this study, we built a DNA six-helix nanostructure based on a group of designed oligonucleotides. Its stability and cellular uptake efficiency were studied. The six-helix nanostructure is more stable and has much higher uptake rate compared to single,strand DNA. The experimental results proved it might be a promising drug deliver.

关 键 词：DNA六螺旋 细胞摄取效率 RAW264.7细胞 MCF-7细胞 HELA细胞 

分 类 号：Q523[生物学—生物化学] TB383.1[一般工业技术—材料科学与工程]