双强度、频度电针对血管性痴呆大鼠海马CA1区β淀粉样蛋白1-40、精氨酸加压素及学习记忆的影响  

Influence of Electroacupuncture Stimulation with Different Intensities and Therapeutic Intervals on Learning-memory Ability and Expression of Aβ1-40 and Arginine Vasopressin Genes in the Hippocampal CA 1 Region in VD Rats

作　　者：蒋灵鸽 张华伟[1] 张拯[1] 邵瑛[1] JIANG Ling-ge ZHANG Hua-wei ZHANG Zheng SHAO Ying(Clinical Medical College of Acu-moxibustion Reha bilitation, Guangzhou University of Chinese Medicine, Guangzhou 510405, China)

机构地区：[1]广州中医药大学针灸康复临床医学院,广州510405

出　　处：《针刺研究》2017年第1期20-24,49,共6页Acupuncture Research

基　　金：中国博士后科学基金第55批面上资助项目(No.2014M550997)

摘　　要：目的:探讨双强度、频度参数交叉配对电针对血管性痴呆(VD)大鼠海马CA 1区β淀粉样蛋白1-40(Aβ1-40)、精氨酸加压素(AVP)及学习记忆的影响。方法:SD大鼠随机抽取8只为假手术组,余大鼠采用4-VO制造血管性痴呆动物模型,造模后筛选出合格的大鼠分为模型组、电针1组(强度0.5mA,每5d治疗1次)、电针2组(强度1.5mA,每5d治疗1次)、电针3组(强度0.5mA,每天治疗1次)、电针4组(强度1.5mA,每天治疗1次),每组8只。电针组针刺"百会""大椎"穴,共治疗10次。运用荧光定量PCR法检测大鼠海马CA 1区Aβ1-40、AVP基因表达,Morris水迷宫测试各组大鼠学习记忆能力。结果:模型组与假手术组比较,平均逃避潜伏期与首次跨越平台时间均延长,2min内跨越平台次数减少,Aβ1-40基因相对表达量增加,AVP基因相对表达量减少(P<0.05)。各电针组与模型组比较,平均逃避潜伏期与首次跨越平台时间均缩短,2min内跨越平台次数增多,Aβ1-40基因相对表达量减少,AVP基因相对表达量增加(P<0.05)。各电针组间进行比较,同频度不同强度时,高强度组(电针2组、电针4组)较低强度组(电针1组、电针3组)大鼠的学习记忆能力强,海马CA 1区Aβ1-40基因相对表达量减少、AVP基因相对表达量增加(P<0.05);同强度不同频度时,高频度组(电针3组、电针4组)较低频度组(电针1组、电针2组)大鼠的学习记忆能力强,海马CA 1区Aβ1-40基因相对表达量减少、AVP基因相对表达量增加(P<0.05)。结论:电针可有效地改善VD大鼠学习记忆能力,其机制可能为电针抑制海马CA 1区Aβ1-40、促进AVP的基因表达;针效高强度优于低强度,高频度优于低频度,高强度与高频度配对治疗方案最优。Objective To observe the influence of electroacupuncture（EA）stimulation with different electrical current intensities and therapeutic intervals on learning-memory ability and expression ofβ-amyloid peptide Aβ1-40 and arginine vasopressin（AVP）genes in the hippocampal CA 1region in vascular dementia（VD）rats,so as to provide evidence for treatment of VD.Methods A total of 48 male SD rats were randomly divided into sham,model,0.5mA-5d-EA,1.5mA-5d-EA,0.5mA-1d-EA and 1.5 mA-1d-EA groups（n=8in each group）.The VD model was established using modified 4-vessels occlusion method.EA（alternative 2Hz/15 Hz,0.5mA,1.5mA）was applied to＂Baihui＂（GV 20）and＂Dazhui＂（GV 14）for 30 min,once a day（1d）or once every 5dfor 10 times.The learning-memory ability was detected using Morris water maze tests（place navigation task and spatial probe trials）,and the expression of Aβ1-40 and AVP genes in hippocampal CA 1region was determined using real time-PCR.Results In comparison with the sham group,the average escape latency of place navigation task,and the duration for crossing the target-platform for the 1st time（spatial navigation task）were significantly increased（P0.05）,and the times to cross the target-platform within 2min（spatial probe trials）were significantly decreased in the model group（P0.05）,suggesting a reduction of learning-memory ability.The expression level of Aβ1-40 mRNA was remarkably increased（P0.05）,and that of AVP mRNA notably decreased in the model group（P0.05）.Following EA intervention,the increased escape latency and the duration of crossing the target-platform for the 1st time and the increased Aβ1-40 mRNA expression,and the decreased target-platform crossing times within 2min as well the down-regulated AVP mRNA expression level were reversed in the 4EA groups compared with the model group（P0.05）.The therapeutic effects of higher stimulating intensity（1.5mA-1d-EA and1.5mA-5d-EA）were markedly superior to those of lower intensity（0.5mA-1d-EA and

关 键 词：血管性痴呆 电针 电针强度/频度 Β淀粉样蛋白1-40 精氨酸加压素 学习记忆能力 

分 类 号：R338.64[医药卫生—人体生理学]