电针对围绝经期抑郁症大鼠下丘脑-垂体-卵巢轴的影响  

Effect of Electroacupuncture on Hypothalamus-Pituitary-Ovary(HPO)Axis in Rats with Perimenopausal Depression

作　　者：蒋希荣 任路[1] 李春日[1] JIANG Xi-rong REN Lu LI Chun-ri(School of Acu moxibustion and Tuina, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, China)

机构地区：[1]辽宁中医药大学针灸推拿学院,沈阳110847

出　　处：《针刺研究》2017年第1期45-49,共5页Acupuncture Research

基　　金：国家自然科学基金项目(No.81373718);国家重点基础研究发展计划("973"计划;No.2010CB 530401973);辽宁省高等学校优秀人才支持计划(No.LR 2014027)

摘　　要：目的:观察电针对围绝经期抑郁症大鼠下丘脑-垂体-卵巢(HPO)轴激素及血清β-内啡肽(β-EP)的影响,探讨针刺调节围绝经期抑郁症大鼠的神经内分泌机制。方法:雌性SD大鼠60只,随机分为空白组、假手术组、模型组、西药组和电针组,采用双侧卵巢切除和慢性不可预见刺激结合建立围绝经期抑郁症大鼠模型。电针组电针"百会"、双侧"肾俞"、双侧"三阴交",西药组使用盐酸氯米帕明灌胃,均每日治疗1次,连续治疗28d。观察大鼠阴道涂片、糖水偏嗜率,运用酶联免疫吸附法测定血清雌二醇(E2)、黄体生成素(LH)、促性腺激素释放激素(GnRH)、β-EP的含量。结果:与空白组相比,模型组及假手术组糖水偏嗜率明显减小(P<0.05);与模型组相比,电针组及西药组糖水偏嗜率升高(P<0.05)。模型组血清E2、β-EP含量较空白组和假手术组明显降低(P<0.05),LH、GnRH明显升高(P<0.05);与模型组比较,电针组及西药组血清E2、β-EP含量明显升高(P<0.05),LH、GnRH明显降低(P<0.05)。结论:针刺能够良性调节围绝经期抑郁症大鼠紊乱的HPO轴功能,改善围绝经期抑郁症状。Objective To observe the effect of electroacupuncture（EA）on the hormones derived from the hypothalamus-pituitary-ovary（HPO）axis,so as to explore the neuroendocrine mechanism induced by EA on rats with perimenopausal depression disorder.Methods Sixty female sprague-dawley rats were randomly divided into blank control group,model group,sham-operation（sham）group,clomipramine group,and electroacupuncture（EA）group,with 12 rats in each group.Perimenopausal depression model was established by bilateral ovariectomy combined with chronic unpredictable stimulation.The EA group received continuous treatment at＂Baihui＂（GV 20）,＂Shenshu＂（BL 23）and ＂Sanyinjiao＂（SP 6）once a day for 28 days.Estrous cycle and sucrose preference test were monitored,and serum estradiol（E2）,luteinizing hormone（LH）,gonadotropin releasing hormone（GnRH）,andβ-endorphin（β-EP）were detected by ELISA.Results Compared to the blank control group,sugar water consumpution rates decreased in the model group and sham group（P0.05）.Compared to the blank group and sham group,the serum LH and GnRH levels increased（P0.05）,companied with lower serum E2andβ-EP levels in the model group（P0.05）.Compared to the model group,sugar water consumpution rates increased in the clomipramine group and EA group（P0.05）,both were companied with decreased serum LH and GnRH levels（P0.05）,and higher serum E2andβ-EP levels（P0.05）.Conclusion Electroacupuncture can relieve the symptoms of rat with perimenopausal depression by regulating the hormone secretion in HPO axis.

关 键 词：电针 围绝经期抑郁症 下丘脑-垂体-卵巢轴 Β-内啡肽 

分 类 号：R245.97[医药卫生—针灸推拿学]