针刺不同穴方对便秘小鼠肠运动的影响  

Effect of Manual Acupuncture Stimulation of Single Acupoint or Acupoints Combination on Intestinal Movement in Slow Transit Constipation Mice

作　　者：高纺[1] 盛红梅[1] 张田宁[1] 吴生兵[2] 曹健[3] 周美启[3] GAO Fang SHENG Hong-mei ZHANG Tian ning WU Sheng-bing CAO Jian ZHOU Mei-qi(Clinical Medical College Center for Experimental Research Section of Science and Technology, Anhui University of Traditional Chinese Medicine, Hefei 230038, China)

机构地区：[1]安徽中医药大学中医临床学院,合肥230038 [2]安徽中医药大学科研实验中心,合肥230038 [3]安徽中医药大学科技处,合肥230038

出　　处：《针刺研究》2017年第1期62-66,75,共6页Acupuncture Research

基　　金：国家重点基础研究发展计划("973"计划)项目(No.2011CB 505205)

摘　　要：目的:比较单穴之间、单穴与相应穴组之间、四单穴组方与相应穴组之间治疗便秘的疗效差异,为临床治疗便秘选穴组方筛选提供实验依据。方法:昆明小鼠190只,每组10只,分别就单穴组、单穴与相应穴组、四单穴组方与相应穴组的比较进行实验设计,均分别设有正常组和模型组做对照。采用灌胃给予复方地芬诺酯建立小鼠便秘模型。模型复制成功后,各治疗组分别选取"曲池""上巨虚""天枢"和"大肠俞"进行针刺。其中,单穴间比较选取4个穴位的单侧,单穴及相应穴组间比较选取4个穴位的双侧以及"天枢"+"大肠俞""曲池"+"上巨虚"的单侧,四单穴组方与相应穴组间比较选取"天枢"+"大肠俞""曲池"+"上巨虚"的双侧以及4个穴位的单侧同时针刺,留针20min,共针刺7次。以上均观察针刺对便秘模型小鼠首次排便时间、12h排便粒数和肠道推进率的影响。结果:1与正常组比较,3个模型组小鼠首次排便时间、12h排便粒数和肠道推进率的差异均具有统计学意义(P<0.01)。2与模型组比较,各单穴(单侧)组小鼠首次排便时间显著缩短(P<0.05),12h排便粒数明显增多(P<0.05),肠道推进率明显提高(P<0.01)。3与模型组比较,各单穴(双侧)组及天枢+大肠俞组、曲池+上巨虚组首次排便时间显著缩短(P<0.05),12h排便粒数明显增加(P<0.05),肠道推进率明显提高(P<0.05),且天枢+大肠俞组与曲池+上巨虚组均优于相应的单穴(双侧)组(P<0.05)。4与模型组比较,四单穴组方组及曲池+上巨虚组、天枢+大肠俞组小鼠首次排便时间显著缩短(P<0.05),12h排便粒数明显增加(P<0.05),肠道推进率明显提高(P<0.05),且四单穴组方组优于天枢+大肠俞组及曲池+上巨虚组(P<0.05)。结论:1针刺各单穴均对治疗慢传输型便秘具有明显改善作用;2针刺穴组的效应优于相应单穴双侧组,提示穴位间具有协同作用;3针刺四单穴组方的效应优于相应双穴组,提�Objective To observe the effect of acupuncture stimulation of different acupoints or acupoint groups on intestinal locomotor in slow transit constipation（STC）mice,so as to provide experimental evidence for demonstrating the synergic function of acupoint combination.Methods A total of 190 Kunming mice were randomized into 19groups：normal control,model,uni-＂Quchi＂（LI 11）,uni-＂Shangjuxu＂（ST 37）,uni-＂Tianshu＂（ST 25）,and uni-＂Dachangshu＂（BL 25）groups,for analyzing the effect of single acupoint;normal control,model,uni-ST 25＋uni-BL 25,uni-LI 11＋uni-ST 37,bi-ST 25,bi-BL 25,bi-LI11,and bi-ST 37 groups,for comparing the effect between single acupoint and two acupoints combination;normal control,model,bi-ST 25＋bi-BL 25,bi-LI 11＋bi-ST 37,and uni-LI 11＋uni-ST 37＋uni-ST 25 ＋uni-BL 25 groups,for comparing the effect between four acupoints and two acupoints combinations（10mice/group）.The STC model was established by intragastric administration of compound diphenoxylate suspension（1mg/100g）,once daily for 2days.Manual acupuncture needle stimulation（200twirling times/min for 1min）was applied to the abovementioned unilateral or bilateral acupoints twice daily for 7times.The first defecation time,the defecation grain number in 12 hand the intestinal propulsion rate were determined using activated carbon gavage.Results Compared with the normal group,the first defecation time was significantly increased in the STC model group（P0.01）,while the 12 hdefecation grain number and the intestinal propulsion rate were considerably decreased in the model group（P0.01）.After manual acupuncture intervention,the first defecation time was notably shortened in the uni-LI 11,uni-ST37,uni-ST 25,uni-BL 25,bi-ST 25,bi-BL 25,bi-LI 11,bi-ST 37,uni-ST 25＋uni-BL 25,uni-LI 11＋uni-ST 37,bi-ST 25＋bi-BL25,bi-LI 11＋bi-ST 37 and the uni-ST 25＋uni-BL 25＋uni-LI 11＋uni-ST 37 groups compared with the model group（P0.05）,while both the 12 hdefecation grain number and intestinal pr

关 键 词：针刺 慢传输型便秘 首次排便时间 粪便粒数 小肠推进率 

分 类 号：R245.311[医药卫生—针灸推拿学]