茉莉酸甲酯对明党参植株和悬浮细胞中呋喃香豆素积累的影响  

Effects of methyl jasmonate on accumulation of furanocoumarins in Changium smyrnioides and its suspension cells

作　　者：蔡静[1] 马赟[1] 陈建伟[1] 李祥[1] 周萍[1] 

机构地区：[1]南京中医药大学,江苏南京210023

出　　处：《中国中药杂志》2017年第1期76-82,共7页China Journal of Chinese Materia Medica

基　　金：国家公益性行业科研专项(201407002);国家教育部博士点基金项目(200803150009);江苏高校优势学科建设工程项目(ysxk-2010);江苏省自然科学基金项目(BK20031s07)

摘　　要：佛手酚、佛手柑内酯和花椒毒素为明党参中天然呋喃香豆素成分,具多重药理作用。以明党参栽培品和悬浮培养细胞为材料,研究茉莉酸甲酯处理后呋喃香豆素积累状况和一系列生理生化指标变化。结果显示,茉莉酸甲酯不同程度地促进明党参中各呋喃香豆素合成,柄悬浮细胞中产量高于叶悬浮细胞。于培养周期第10天加入100μmol·L-1茉莉酸甲酯对明党参悬浮细胞作用效果最明显,最适收获时间为处理4 d后,此时柄悬浮细胞中佛手酚,花椒毒素和佛手柑内酯产量分别是未诱导细胞的73.6,1.9,42.7倍。茉莉酸甲酯诱导呋喃香豆素增长的同时抑制细胞活力和生物量积累,激发细胞防御反应。该研究初次系统地探索了茉莉酸甲酯对明党参中呋喃香豆素的诱导效果,并为大量生产3种活性成分和探明茉莉酸甲酯诱导机制奠定基础。Changium smyrnioides is an endangered and endemic medicinal herb in China which contains rich furanocoumarins. Ber- gaptol, bergapten and xanthotoxin are natural furanocoumarins in Ch. smyrnioides, among which bergaptol is mainly contained in in vitro cultures while the latter ones distribute in all organs and cultures of the plant. In this study, methyl jasmonate was used to elicit furanocoumarins in both cultivated plant and suspension cells. The accumulations of biomass and 3 furanocoumarins as well as the ac- tivity of cell, phenylalanine ammonia-lyase and antioxidase were detected. The results showed that methyl jasmonate induced the bio- synthesis of furanocoumarins markedly and suspension cells from petiole produced more furanocoumarins than those from leaf. In the case of suspension cells, the concentration at 100 μmol·L^-1 triggered the highest yield of furanocoumarins and the 10tb day of the culture period was the proper time for treatment. After 4 days the yields of bergaptol, bergapten and xanthotoxin in suspension cells from petiole were enhanced to 2. 83,14. 04,0. 62 mg · L^-1 respectively. The biomass and viability of treated suspension cells de- creased. At the same time, the activity of antioxidase increased, which indicated that methyl jasmonate induced cell defense. In both in vivo and in vitro conditions, cells from petiole seemed to be more sensitive to methyl jasmonate treatment compared to those from leaf. Bergaptol and xanthotoxin mainly accumulated in medium and cell respectively. Bergapten was detected in both cell and medium. The elieitation treatment only enormously affected the yields but did not significantly involve the distributions of 3 furanocoumarins. This is the first systematic study focusing on the elicitation effects of methyl jasmonate and a series of changes which lead to the increase of furanoeoumarins in Ch. smyrnioides cell suspension cultures. Methyl jasmonate appears to be an effective elicitor in the research and further efforts should be made to reveal the mechan

关 键 词：明党参 呋喃香豆素 茉莉酸甲酯 诱导 

分 类 号：S567.53[农业科学—中草药栽培]