右美托咪定对LPS诱导星形胶质细胞MCP-1分泌的影响  

Effect of dexmedetomidine on lipopolysaccharide-induced production of MCP-1 mRNA in rat astrocytes

作　　者：朱翔[1] 戴林[2] 姜保春[3] 杨建平[1] 

机构地区：[1]苏州大学附属第一医院麻醉科,苏州215006 [2]南通大学附属医院风湿免疫科,南通226001 [3]南通大学航海医学研究所,南通226001

出　　处：《安徽医科大学学报》2017年第1期90-94,共5页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81400915);南通大学附属医院转化医学基地科研项目(编号:TDFzh2014013)

摘　　要：目的研究右美托咪定对脂多糖(LPS)诱导大鼠星形胶质细胞MCP-1 mRNA分泌的影响。方法培养原代大鼠星形胶质细胞,待诱导分化成熟,免疫组化检测α-2A肾上腺受体的表达;不同浓度LPS刺激星形胶质细胞,观察LPS浓度与MCP-1 mRNA表达的量效关系;随后将细胞随机分为6组:对照组(control组)、LPS(200 ng/ml)刺激组(LPS组)、右美托咪定(DEX)500 ng/ml孵育组(DEX组)、DEX(10、100、500 ng/ml)+LPS(200 ng/ml)组[DEX(10、100、500 ng/ml)+LPS组],荧光实时定量PCR检测各组MCP-1mRNA的表达。结果α-2A肾上腺受体在星形胶质细胞表达,与细胞标志物GFAP完全共标;不同浓度LPS刺激可引起MCP-1剂量依赖性的表达增加(F(6,41)=289.35,P<0.001),LPS刺激引起MCP-1 mRNA表达量增加的ED50为150.8 ng/ml,ED95为344.1 ng/ml,r=0.86;与LPS组相比,不同浓度右美托咪定均可抑制LPS刺激引起的星形胶质细胞MCP-1 mRNA升高(F(5,21)=454.15,P<0.001)。结论右美托咪定可抑制LPS刺激大鼠星形胶质细胞MCP-1 mRNA的表达,该作用可能是其减轻疼痛的机制之一。Objective To observe the effect of dexmedetomidine （DEX） on lipopolysaccharide - induced produc- tion of MCP-1 mRNA in rat astrocytes. Methods Primary rat astrocytes were double immunofluoreseence stained of α-2A drenoeeptors（α-2AR） and glial fibrillary acidic protein（ GFAP; an astrocytic marker）. Different concentrations of LPS were chosen to stimulate astrocytes, then the induced mature astrocytes were divided into six groups, control group （Opti-MEM 500μl treaded for three hours）, LPS 200 ng/ml group（ LPS 200 ng/ml treaded for three hours） , DEX 500 ng/ml group（only DEX treated for three hours） , other groups were incubated with DEX 10, 100 and 500 ng/ml respectively for half an hour and then all treated with 200 ng/ml LPS for three hours. The levels of MCP-1 mRNA were measured by Real-time PCR. Results Expression of α-2A adrenergic receptors（α-2AR） was in astrocytes and double immunofluorescence showed that α-2AR were colocalized with （a） glial fibrillary acidic protein （GFAP; an astrocytic marker）. The expression level of MCP-1 mRNA was increased in a dose-dependent manner with LPS treatment（ F〈6,41） = 289.35 ,P 〈 0. 001 ）, ED50 = 150. 8 ng/ml, ED95 = 344. 1 ng/ml, r = 0. 86. DEX at 10,100 and 500 ng/ml signifcantly inhibited the release of MCP-1 mRNA compared with LPS 200 ng/ml group（F（5.21）= 454. 15, P 〈 0. 001 ）. Conclusion DEX is a potent dose-dependent suppressor of LPS-induced MCP-1 in activated astrocyte cells and may be one of the mechanisms for the reduction of the pain.

关 键 词：右美托咪定 MCP-1 星形胶质细胞 α-2A肾上腺受体 

分 类 号：R392.12[医药卫生—免疫学] R363.1[医药卫生—基础医学]