丹酚酸A对大鼠脑缺血再灌注损伤后胞浆型磷脂酶A2表达的影响及机制研究  

Effect of salvianolic acid A on expression of cytosolic phospholipase A2 in rats following cerebral IR injury and its mechanism

作　　者：彭潇[1] 郑丽云[1] 方世记[1] 邬至平[1] 蔡学礼[1] 黄良通[1] PENG Xiao ZHENG Li-yun FANG Shi-ji et al(Department of Neurology ,Fifth Affiliated Hospital of Wenzhou Medical University, Lishui 323000 ,Zhejiang Province ,Chin)

机构地区：[1]温州医科大学附属第五医院神经内科,丽水323000

出　　处：《中华老年心脑血管病杂志》2017年第2期195-198,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：浙江省科技计划项目(2016C37128)

摘　　要：目的探讨丹酚酸A对大鼠脑缺血再灌注损伤后胞浆型磷脂酶A2(cytosolic phospholipase A2,cPLA2)表达的影响及其机制。方法将SD大鼠65只,随机分为对照组,模型组、丹酚酸A组和联合组(LY294002+丹酚酸A)。分别于再灌注后0、8、24、48h取梗死脑组织,用TUNEL检测脑细胞凋亡,免疫组织化学检测cPLA2表达,Western blot检测蛋白激酶B(Akt)、磷酸化Akt在脑组织表达水平。结果模型组和联合组0、8、24、48h海马CAl区TUNEL阳性细胞和cPLA2表达较对照组明显增高,脑组织磷酸化Akt表达较对照组明显降低(P<0.05)。丹酚酸A组0、8、24、48h脑组织磷酸化Akt表达较模型组明显增高,TUNEL阳性细胞和cPLA2表达较模型组明显降低[(10.1±5.3)个/mm^2 vs(12.8±4.7)个/mm^2、(14.6±6.2)个/mm^2 vs(30.1±7.5)个/mm^2、(19.7±6.5)个/mm^2 vs(73.8±11.4)个/mm^2、(22.8±9.6)个/mm^2 vs(94.6±15.2)个/mm^2,(13.6±3.1)个/mm^2 vs(41.3±3.6)个/mm^2、(14.8±4.7)个/mm^2 vs(62.5±6.9)个/mm^2、(16.6±7.1)个/mm^2 vs(72.3±9.6)个/mm^2、(17.0±5.3)个/mm^2 vs(83.2±11.7)个/mm^2,P<0.05]。结论丹酚酸A通过Akt信号通路,降低脑缺血再灌注后脑组织中cPLA2表达,减少脑细胞凋亡,发挥细胞保护作用。Objective To study the effect of salvianolic acid A on expression of cytosolic phospholipase A2（cPLA2）in rats following cerebral IR injury and its mechanism.Methods Sixty-five SD rats were randomly divided into control group,model group,salvianolic acid A treatment group and LY294002＋salvianolic acid A treatment group（20in each group）.Brain tissue samples were taken from ischemic stroke rats at 0,8,24 and 48hafter reperfusion.Apoptosis was assayed by TUNEL,expressions of cPLA2,Akt and phosphorylated Akt were detected by Western blot withimmunohisto chemical staining.Results The number of TUNEL-positive cells was greater and the expression level of cPLA2 was significantly higher while the expression level of phosphorylated Akt was significantly lower in model group and LY294002＋salvianolic acid A treatment group than in control group at 0,8,24,48 hafter reperfusion（P〈0.05）.The expression level of phosphorylated Akt was significantly higher while the number of TUNEL-positive cells was significantly smaller and the expression level of cPLA2 was significantly lower in salvianolic acid A treatment group than in model group at 0,8,24,48 hafter reperfusion（P〈0.05）.ConclusionSalvianolic acid A inhibits the expression of cPLA2 and reduces the apoptosis through the Akt pathway,thus playing a role in protecting cells in rats following IR injury.

关 键 词：脑缺血 磷脂酶A2 胞质型 再灌注损伤 蛋白激酶类 

分 类 号：R285.5[医药卫生—中药学]