丙泊酚对大鼠肺动脉平滑肌细胞增殖/凋亡的影响及其分子机制  

Study of Propofol on Growth and Apoptosis of Rat Pulmonary Artery Smooth Muscle Cells and the Related Mechanisms

作　　者：王鹏[1] 郝伟[1] 张颖[1] 任立洁[1] 张艳莉[1] 单世民[1] 

机构地区：[1]天津市第五中心医院麻醉科,天津300450

出　　处：《中国中西医结合外科杂志》2017年第1期57-60,共4页Chinese Journal of Surgery of Integrated Traditional and Western Medicine

基　　金：天津市卫生局科技基金项目(2014ZK016)

摘　　要：目的:研究丙泊酚对大鼠肺动脉平滑肌细胞增殖和凋亡的影响及其相关分子机制。方法:将原代大鼠肺动脉平滑肌细胞接种在培养孔板,并随机分为对照组(C组)、丙泊酚2μg/m L组(F1组)、丙泊酚5μg/m L组(F2组),处理时间为24、48、72 h,收集对应细胞进行细胞增殖、周期和凋亡的检测,并收集对应的RNA和蛋白,利用荧光定量PCR和免疫印迹Western Blot检测细胞增殖/凋亡标志物PCNA、Bax的表达。结果:丙泊酚对大鼠肺动脉平滑肌细胞增殖的抑制率及凋亡的促进率具有时间和浓度依赖性;MTT实验证明,5μg/m L组的丙泊酚可显著抑制大鼠肺动脉平滑肌细胞增殖,同时抑制PCNA的m RNA和蛋白表达(二者下降比率为40%);流式细胞术证实,5μg/m L丙泊酚可诱导大鼠肺动脉平滑肌细胞的周期抑制和凋亡,并上调促凋亡蛋白BAX表达(上调比率为3.6倍)。结论:5μg/m L浓度的丙泊酚在体外可有效抑制大鼠肺动脉平滑肌细胞增殖并诱导细胞凋亡。ObjectiveTo study the effects and related molecular mechanisms of propofol on growth andapoptosis of rat pulmonary artery smooth muscle cells.MethodsRat pulmonary artery smooth muscle cellswere seeded in culture plates and treated with propofol（0, 2, 4~5 μg/m L） for 24 and 48 hours. Then, cells wereharvested for measuring cell growth, cell cycle and apoptosis rates. RNA and protein were also extracted for re-al-time PCR and western blotting analysis of PCNA and BAX expression, respectively.ResultsPropofolcould significantly induce growth inhibition and apoptosis in rat pulmonary artery smooth muscle cells at a con-centration- and time-dependent manner. MTT assay showed that 5 μ g/m L propofol could suppress growth ofrate pulmonary artery smooth muscle cells, and reduce the m RNA and protein expression of PCNA（both at anapproximate 40% reduction rate）. Also, flow cytometry showed that propofol at high dose could induce cell cyclearrest, elevate the apoptosis rate of pulmonary artery smooth muscle cells, and meanwhile up-regulate the BAXprotein expression in the treated groups（at an approximate 3.6-fold up-regulation）.ConclusionPropofol at5 μg/m L could suppress growth and promote apoptosis of rat pulmonary artery smooth muscle cells in vitro.

关 键 词：丙泊酚 肺动脉平滑肌细胞 增殖 凋亡 

分 类 号：Q95-33[生物学—动物学] R971.3[医药卫生—药品]