基于纳米金荧光猝灭体系的汗潜指纹示踪研究  

Preliminary Study on Application of Gold Nanoparticles-Fluorescence Quenching System in Fingerprint Detection

作　　者：丁冲[1] 徐帅[1] 王利华[1] 吕笑笑[2] DING Chong XU Shuai WANG Li-hua LU Xiao-xiao(Key Laboratory of Catalysis and Materials Science of the State Ethnic Affair Commission Ministry of Education, Hubei Province, South-Central University for Nationalities ,Wuhan 430074 Environmental Science and Chemistry College, Hunan University, Changsha 410082)

机构地区：[1]中南民族大学化学与材料科学学院催化材料科学湖北省暨国家民委-教育部共建重点实验室,湖北武汉430074 [2]湖南大学环境科学与化学学院,湖南长沙410082

出　　处：《分析科学学报》2017年第1期17-20,共4页Journal of Analytical Science

基　　金：中央高校基本科研业务费(No.CZY13028);国家大学生创新创业计划(No.201411798004)

摘　　要：本文构建了一种新的汗潜指纹示踪技术,该技术将纳米金与荧光素在合适条件下混合,形成稳定的纳米金-荧光素猝灭体系。该体系基于纳米金荧光与目标指纹结合后,由于纳米金与指纹中的Cl-结合从而在目标指纹处恢复荧光显现出指纹轮廓。通过在不同类型的材料上进行效果检验。发现在非渗透性材料、渗透性材料、高聚物材料中纳米金荧光猝灭体系对于汗潜指纹均有较为良好的示踪效果。This study introduces a remarkable method of fingerprints detection using gold nanoparticlesfluorescein quenching system.The system relies on the ability of gold nanoparticles（AuNPs）species to cause rapid quenching of specific fluorescein in an aqueous dispersion containing alcohol.This quenching occurs via fluorescence resonance energy transfer（FRET）and surface energy transfer（SET）when the fluorescein is attached to the surface of AuNPs.The system forms only with 10-5 mol/L fluorescein and AuNPs of a particular size（about average less than 20 nm diameter）,and creates efficient and immediate quenching.With the introduction of chloride ions（Cl-）,which have a higher binding affinity to the surface of Au and can replace the fluorescein on the surface of AuNPs,the AuNPs-fluorescein quenching system can be destructed rapidly.Because fingerprints contain chloride ions,the simple and cost-effective system provides rapid and simple detection of fingerprints,it will reemerge stronger fluorescence where the latent fingerprints exist,and thus latent fingerprints show up.The system has been applied in varius materials including non-permeable material,permeable material and polymer material,and good results have been obtained.

关 键 词：纳米金 荧光猝灭体系 汗潜指纹 示踪 

分 类 号：O657.39[理学—分析化学]