离子色谱法测定蓝藻培养液中有机酸和无机阴离子  

Determination of Organic Acids and Inorganic Anions in Fermentation of Cyanobacterial with Ion Chromatography

作　　者：黄海兰[1] 王鑫[1,2] 法芸[2] 张经华[3] 王宗花[1] HUANG Hai-lan WANG Xin FA Yun. ZHANG Jing-hua WANG Zong -hua(College of Chemistry and Chemical Engineering ,Qingdao University, Qingdao 26607 Key Laboratory of Bio fueis ,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences ,Qingdao 266101 Beijing Centre for Physical ＆ Chemical Analysis ,Beijing 100049)

机构地区：[1]青岛大学化学科学与工程学院,山东青岛266071 [2]中国科学院青岛生物能源与过程研究所,生物燃料重点实验室,山东青岛266101 [3]北京市理化分析测试中心,北京100049

出　　处：《分析科学学报》2017年第1期32-36,共5页Journal of Analytical Science

基　　金：国家自然科学基金(No.21405166);科技部重大专项(No.2012YQ090229)

摘　　要：建立了一种利用阴离子交换色谱柱分离,电导检测器同时测定17种有机酸和7种阴离子的方法。采用Ionpac AS11-HC阴离子交换柱,KOH溶液作为淋洗液,梯度洗脱,流量为1.0mL/min,柱温为30℃,进样体积为25μL。24种分析物测定结果的相对标准偏差在0.31%~2.67%之间,线性相关系数均大于0.996,平均加标回收率在80.3%~108.5%之间。该方法可用于微生物培养液中有机酸和阴离子的同时测定。A method for the simultaneous determination of 17 organic acids and 7inorganic anions by using ion chromatography with suppressed conductivity detector were established.Separation was performed on an IonPac AS11-HC column with 1.0mL/min eluent of KOH and the column temperature was 30℃.The results showed the relative standard derivations of 24 analytes were ranged from 0.31%to 2.67%.The correlation coefficients were greater than 99.6%.The mean recoveries observed were between 80.3% and 108.5%.This method is rapid and accurate,which has been applied to simultaneous determination of organic acids and inorganic anions in microbial cultures with satisfactory results.

关 键 词：阴离子交换色谱 电导检测器 有机酸 阴离子 

分 类 号：O657.75[理学—分析化学]