溴百里酚蓝/多壁碳纳米管修饰玻碳电极测定冬虫夏草中腺嘌呤  

MWCNTS/BTB Modified Glassy Carbon Electrode Determination of Adenine in CordycepsSinensis

作　　者：冯宇婷 申贵隽[1] 黄慧娟[1] 

机构地区：[1]大连大学环境与化学工程学院,大连116622

出　　处：《理化检验（化学分册）》2017年第1期60-63,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：用循环伏安法(CV)于磷酸盐缓冲溶液中在-0.8~1.6V电位间扫描15周,使溴百里酚蓝(BTB)聚合于玻碳电极表面,制得BTB/GCE电极。另取经羰基化的多壁碳纳米管10mg分散于10 mL DMF中,并将此分散液适量滴于BTB/GCE电极表面,于红外灯中烘干后即得MWCNTS/BTB/GCE修饰电极,用于测定冬虫夏草中腺嘌呤含量的传感器。通过CV研究表明:该传感器在pH 6.8的磷酸盐缓冲溶液中,扫描速率为100mV·s-1的条件下对腺嘌呤的响应明显,且腺嘌呤的质量浓度在0.05~1.0g·L-1范围内与相应的氧化峰电流呈线性关系,其检出限(3s)为0.025g·L-1。该方法用于5个不同批次的样品的分析,测得腺嘌呤含量与其标示量相符,测定值的相对标准偏差(n=5)在0.8%~2.0%之间。Bromothymol blue （BTB） was polymerized on the surface of GCE in a phosphate buffer solution by CV in the potential range of -0. 8 to 1.6 V, with scanning for 15 cycles, to form BTB modified GCE. A portion of 10 mg of carbonylated MWCNTS was dispersed in 10 mL of DMF, and an appropriate amount of the dispersion was dropped on the surface of the BTB/GCE, forming the modified electrode of MWCNTS/BTB/GCE, which was dried under ultra-red lamp. As shown by the CV study, significant response of adenine at the MWCNTS/BTB/GCE was observed in phosphate buffer at pH 6.8 with scanning rate of 100 mV · s^-1. It was proved that the modified GCE was suitable to be used as a sensor for CV determination of adenine in Cordyceps sinensis. Linear relationship between values of oxidative peak current and mass concentration of adenine was obtained in the range of 0. 05- 1.0 g · L^- 1, with detection limit （3s） of 0. 025 g· L^- 1. 5 samples taken from different batches were taken and analyzed by the proposed method, giving results in consistency with the labelled values. Values of RSDs （n= 5） found were in the range of 0. 8 %-2.0 %.

关 键 词：循环伏安法 MWCNTS/BTB/GCE修饰电极 腺嘌呤 冬虫夏草 

分 类 号：O657.1[理学—分析化学]