AKT转染羊水间充质干细胞对兔缺血再灌注损伤心肌的修复作用  

Cardiac repair effect of AKT transfected amniotic fluid mesenchymal stem cells on ischemic reperfusion injured myocardium in rabbits

作　　者：宋蕾[1] 张松[1] 王燕[1] 姜珊[1] 李研研[1] 刘伟[1] 卢英民[2] SONG Lei ZHANG Song WANG Yan JIANG Shan LI Yan-yan LIU Wei LU Ying-min(Department of Cardiology, Affiliated Xinhua Hospital, Medical College, Shanghai Jiaotong University, Shanghai, 200092, Chin)

机构地区：[1]上海交通大学医学院附属新华医院心内科,上海市200092 [2]上海交通大学医学院附属新华医院崇明分院

出　　处：《心血管康复医学杂志》2017年第1期68-73,共6页Chinese Journal of Cardiovascular Rehabilitation Medicine

基　　金：国家自然科学基金资助项目(81170124)~~

摘　　要：目的:探讨AKT基因转染的羊水间充质干细胞移植于兔缺血再灌注损伤模型后对缺血再灌注损伤心肌的修复作用。方法:构建AKT过表达慢病毒载体转染羊水间充质干细胞;新西兰兔随机分为A组:低糖型培养基(LDMEM)组、B组:羊水间充质干细胞(AF-MSC)组、C组:AKT-转染羊水间充质干细胞(AKT-AFMSC)组。建立兔心肌缺血再灌注损伤模型。再灌注开通前在梗死区及周边心肌外膜按分组直接注射0.2ml:L-DMEM、AFMSC、AKT-AFMSC。术后21d比较各组左室舒张末期内径(LVEDd)、左室短轴缩短率(LVFS)、左室射血分数(LVEF),比较坏死区范围及病理变化。结果:与L-DMEM组、AF-MSC组比较,AKT-AFMSC组LVEDd[(16.37±0.84)mm、(15.06±1.01)mm比(13.51±0.85)mm]显著减小,LVFS[(29.18±2.36)%、(33.65±2.81)%比(36.89±3.02)%]、LVEF[(58.62±3.47)%、(67.42±3.03)%比(72.02±2.89)%]显著提高(P<0.05或<0.01)。AKT-MSC组梗死面积明显小于L-DMEM组和AF-MSC组[(18.1±1.2)%比(37.3±2.1)%、(26.6±0.7)%,P<0.01];AKT-AFMSC组病理损伤较A组,B组明显减轻。结论:在缺血再灌注损伤心肌中,AKT转染羊水间充质干细胞具有较羊水间充质干细胞更强的修复损伤心肌、改善心肌功能的作用。Objective： To explore cardiac repair effect of AKT gene transfected amniotic fluid mesenchymal stem cells （AFMSC） transplantation on ischemic reperfusion injured myocardium in rabbit model. Methods： AKT overexpressed lentiviral vector was established to transfect AFMSC; New Zealand rabbits were randomly divided into low glucose DMEM （L-DMEM） group （group A）, AFMSC group （group B） and AKT transfected AFMSC group （AKT- AFMSC group, group C）. Then rabbit myocardial ischemia reperfusion injury model was established. Before reperfusion, 0.2ml L-DMEM, AFMSC and AKT-AFMSC was directly injected into infarct area and surrounding myocardial epicardium in corresponding group. On 21 days after operation, left ventricular end-diastolic dimension （LVEDd）, left ventricular shortening fraction （LVFS）, left ventricular ejection fraction （LVEF）, infarct size and pathological changes were compared among three groups. Results： Compared with L-DMEM group and AF-MSC group, there was significant reductions in LVEDd [ （16.37 ± 0.84） ram, （15.06 ± 1.01） mm vs. （13.51 ± 0.85） mm] and infarct size [ （37.3 ± 2.1） %, （26.6 ± 0.7） % vs. （18.1 ±1.2） %], and significant rise in LVFS [ （29.18 ±2.36）%, （33.65±2.81）% vs. （36.89±3.02）%] andLVEF [ （58.62±3.47）%, （67.42±3.03）% vs. （72.02 ±2.89）%], P〈0.05 or 〈0.01. Pathological injury significantly relieved in AKT-AFMSC group than those of group A and group B. Conclusion： Compared with amniotic fluid mesenchymal stem cells, AKT-transfected amniotic fluid mesenchymal stem cells possesses better effects of repairing injured myocardium and improving myocardial function in ischemic reperfusion injured myocardium.

关 键 词：蛋白质丝氨酸苏氨酸激酶 间质干细胞移植 再灌注损伤 

分 类 号：R542.2[医药卫生—心血管疾病]