替尼泊苷磷脂复合物白蛋白纳米粒的制备与表征  

Preparation and characterization of Teniposide phospholipid complex albumin nanoparticle

作　　者：张宇[1] 和心依[1] 龚涛[1] 

机构地区：[1]四川大学华西药学院,四川成都610041

出　　处：《华西药学杂志》2017年第1期13-15,共3页West China Journal of Pharmaceutical Sciences

摘　　要：目的制备替尼泊苷磷脂复合物白蛋白纳米粒,并表征其理化性质。方法以人血清白蛋白和蛋黄卵磷脂E80为辅料,替尼泊苷为主药,采用超声法制备替尼泊苷磷脂复合物白蛋白纳米粒及其冻干制剂。以粒径和多分散系数(PDI)为主要考察指标来优化纳米粒的处方及制备工艺;用激光粒度分析仪和透射电镜对其形态和结构进行表征;用葡聚糖凝胶柱法测定纳米粒的包封率和载药量。结果成功制备了替尼泊苷磷脂复合物白蛋白纳米粒,平均粒径为182.3±11.7 nm,PDI为0.168±0.02,Zeta电位为-10.75±1.42 m V,包封率为82.27%±2.74%,载药量为4.29%±0.11%;冻干制剂的外观良好,复溶后的粒径和PDI均符合要求。结论所用方法简单新颖,具有较好的应用前景。OBJECTIVE To establish a novel preparation method of Teniposide phospholipid complex albumin nanoparticles and characterize its physicochemical properties. METHODS With human serum albumin and egg yolk lecithin ES0 as exeipients ,Teniposide as principal agent, ultrasonic method was adopted to prepare Teniposide phospholipid complex albumin nanoparticles and its lyophilized preparation. The optimal prescription and preparation process with particle size and polydispersity index（PDI） as the main measure were screened. The morphology and structure of the nanoparticle were characterized by Zetasizer and transmission electron microscope. The encapsulation efficiency and drug loading were determined by Sephadex column method. RESULTS Teniposide phos- pholipid complex albumin nanoparticles were successfully prepared. The average particle size was 182.3 ± 11.7 um with PDI of 0. 168 ±0.02,Zeta potential of - 10.75 ± 1.42 mV, encapsulation efficiency of 82.27% ± 2.74% and drug loading of 4.29% ± 0. 11%. The lyophilized preparation showed good appearance, and kept eligible particle size and PDI after reconstitution. CONCLUSION The method is simple and novel, showing good application prospect.

关 键 词：替尼泊苷 磷脂 复合物 白蛋白 纳米粒 超声法 制备 表征 

分 类 号：R94[医药卫生—药剂学]