胎盘IGF-1的表达及其DNA甲基化与IUGR的相关性分析  

Correlation Analysis between the Expression and DNA Methylation of IGF-1 in Placenta and IUGR

作　　者：李晓娟[1] 于柏峰 吴学礼[3] 张继业[1] 贾彩红[4] 王卓[4] 周俏苗[5] 周红桃[1] 易国辉[6] 符生苗[1] 

机构地区：[1]海南省人民医院中心实验室,海南省细胞与分子遗传转化医学重点实验室,海口570311 [2]美国犹他州大学儿科系,犹他州84101 [3]海口市妇幼保健院检验科,海口570203 [4]中国热带农业科学院热带生物技术研究所,海口571101 [5]海南省人民医院妇产科,海口570311 [6]海南医学院科学实验中心,海口571199

出　　处：《基因组学与应用生物学》2017年第1期7-12,共6页Genomics and Applied Biology

基　　金：海南省自然科学基金(811182)资助

摘　　要：为探究胎盘中胰岛素样生长因子1的浓度和基因甲基化变化与宫内生长受阻(IUGR)发生的关系。选择56名正常妊娠足月分娩的产妇和新生儿为对象,其中27名IUGR患儿,29名正常出生体重儿,收集产妇及新生儿的基本信息和胎盘样本。用实时荧光定量PCR检测IGF-1 m RNA表达量,用BSP分析胎盘IGF-1基因启动子区Cp G位点的甲基化水平。IUGR组IGF-1 m RNA的ΔCT值为(7.483±1.406),对照组为(5.642±1.323),经t检验,t=3.567,p=0.001(p<0.01),提示IUGR患者胎盘IGF m RNA表达低于对照组。胎盘IGF-1启动子区Cp G位点均呈高甲基化状态,两组间平均甲基化率的差异无统计学意义,但两组中男性和女性IGF-1甲基化率存在差异。IGF-1在IUGR的发生中起着重要的作用,一定水平的IGF-1对维持胎儿生长是至关重要的,其表达量的高低均与处于高甲基化状态的胎盘IGF-1的甲基化程度无关,与性别有关。In order to explore the influence of the expression of insulin-like growth factor 1 （IGF- 1） gene and its methylation changes on intrauterine growth retardation 1 0UGR）, we chose 56 full-term delivery of expectant mothers and their newborns as sapmples, among which there were 27 IUGR infants and 29 infants with normal birth weight, and we collected some basic information of these gravidas and infants and their placenta samples. The expression of IGF-1 mRNA in placenta was analyzed by real-time quantitative PCR, and the methylation level of CpG site in IGF-1 promoter region was analyzed by BSP. The ACT value of IGF-1 mRNA from samples of IUGR group （7.483±1.406） was significantly lower than that of control group （5.642± 1.323） and the t test showed that t=3.567,p =0.001 （p 〈0.01）, which indicated that the expression of IGF-1 mRNA in placenta of IUGR patients was lower than the control group. High methylation state of CpG site in IGF-1 promoter region of placenta was observed in both groups and it was not statistically significant, but there were significant differences in methylation state in IGF-1 between female and male infants in the two groups. In conclusion, IGF-1 plays an important role in the occurrence of IUGR, and a certain level of IGF-1 is critical for maintaining the growth of fatal, whereas its expression quantity has no relationship with the methylation level of IGF-1 in placenta of high methylation state but is related to the genders of infants.

关 键 词：胎盘 胰岛素样生长因子 宫内生长受限 DNA甲基化 

分 类 号：R714.2[医药卫生—妇产科学]