基于AFLP技术的菜粉蝶遗传多样性初步分析  

Preliminary Analysis of Genetic Diversity of Pieris rapae Populations Based on AFLP Technique

作　　者：陈晓宁[1] 杨美霞[1] 冯慧[1] 

机构地区：[1]陕西省动物研究所,西安710032

出　　处：《基因组学与应用生物学》2017年第1期117-120,共4页Genomics and Applied Biology

基　　金：陕西省13115重大科技专项(2010ZDKG-54);陕西省科技计划项目(2012KW-21);陕西省科学院科技计划项目(2016K-24)共同资助

摘　　要：本研究选用陕西境内5个采样点采集的30个菜粉蝶个体,用AFLP技术分析了5个种群的遗传多样性。凝胶图经染色和分析发现总位点数为540个,其中504个为多态性位点,36个共有位点,具多态性的条带百分率为93.33%。结果表明:AFLP技术适合应用于菜粉蝶遗传多样性分析;荧光标记法在AFLP分析中具有较高的检测效率;南北坡5个地点种群的遗传多样性分化显示出山脉对菜粉蝶的隔绝作用。In this paper, we selected 30 Pieris rapae individuals from 5 searching sites in Shaanxi which belonged to 5 populations to analyze their genetic diversities by using the AFLP technique. Through staining and analyzing the gel map, we got 540 sites altogether, among which there were 504 polymorphic sites and 36 common sites and the polymorphic percentage was 93.33%. The results showed that AFLP technique was suitable for the genetic diversity analysis of Pieris rapae; Fluorescence labeling method had high detection efficiency in AFLP analysis; Genetic diversities of populations from 5 sites in north and south slopes showed that the mountains had isolation effect towards Pieris rapae.

关 键 词：AFLP 菜粉蝶 遗传多样性 

分 类 号：S433.4[农业科学—农业昆虫与害虫防治]