铁皮石斛黑斑病菌小分子毒素提取及产毒条件优化  

Extraction and Cultural Optimization of Small Molecule Toxins of Pathogen Causing Black Spot Disease from Dendrobium officinale

作　　者：孔琼[1] 袁盛勇[1] 薛春丽[1] 汪正红[1] 李祥[1] 卢丽蓉[1] 明应忠 

机构地区：[1]红河学院生命科学与技术学院,云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661199

出　　处：《西南农业学报》2017年第1期118-121,共4页Southwest China Journal of Agricultural Sciences

基　　金：红河学院校级委托项目(13WT001);红河学院博士启动项目(14bs14);红河学院大学生科技创新基金(SZ1319);红河学院大学生创新创业训练计划项目(DCXL1305);红河学院硕士点植物保护一级学科建设项目

摘　　要：铁皮石斛是一种重要的中草药,随着人工种植面积的扩大和年限延长,铁皮石斛上的各种病害随之而来,其中由链格孢引起的石斛黑斑病日趋严重。本研究选取铁皮石斛黑斑病病原—细极链格孢为供试菌株,采用不同溶剂提取病原小分子毒素,同时对病原小分子毒素的产毒条件进行优化。结果表明,乙酸乙酯的萃取效果最好,且小分子毒素的活性成分主要存在于水相中;当培养基为PSK培养液,温度为25℃,转速为120 r/min,L∶D=12∶12的培养方式全振荡培养7 d时,该病原的小分子毒素活性最强。Dendrobium officinale was a precious traditional Chinese herb. The various diseases affected growth ofDendrobium officinale with growing areas and ages, of which the black spot disease caused by Alternaria tenuissima was more and more serious. The extraction reagents and production conditions of small molecular toxins of A. tenuissima in black spot disease were studied in this paper. The results showed that the best extraction reagents was ethyl acetate, and the active ingredient of toxin was mainly in aqueous phase, the optimal cultural conditions were PSK liquid medium, 25 ℃, 7 day in 120 r/min with photoperiods of L： D = 12： 12, respectively.

关 键 词：铁皮石斛 黑斑病 小分子毒素 

分 类 号：S567[农业科学—中草药栽培]