茎瘤芥(榨菜)抽薹性状相关SSR分子标记的筛选  

Selection of SSR Molecular Markers of Bolting in Tumorous Stem Mustard

作　　者：沈进娟 杨仕伟 刘雪姣 冉广葵 于晓虎 曾胜 张召荣 朱学栋 

机构地区：[1]重庆市渝东南农业科学院,重庆涪陵408099

出　　处：《西南农业学报》2017年第1期188-192,共5页Southwest China Journal of Agricultural Sciences

基　　金：重庆市基础科学与前沿技术研究(一般)项目(cstc2013jcyj A80028);重庆市应用开发(一般)项目(cstc2013yykf A80011);重庆市社会事业与民生保障科技创新专项项目(cstc2015shms-ztzx80005)

摘　　要：茎瘤芥先期抽薹给榨菜产量带来极大损失,是当前影响榨菜产业快速健康发展的主要因素之一。本研究采用茎瘤芥极早抽薹材料‘92’和极晚抽薹材料‘203’配制杂交组合,自交获得F2代群体,根据集团分离分析法,运用600对芸薹属SSR共有引物,进行茎瘤芥抽薹性状基因连锁的分子标记筛选。最终得到1个SSR标记Ol12-D09在早抽薹基因池中扩增出特征条带,而晚抽薹基因池中无特征条带,经F2代单株验证发现与茎瘤芥早抽薹基因紧密连锁,根据Kosambi函数估算其连锁距离为10.9 c M。本研究结果筛选的SSR标记可用于茎瘤芥先期抽薹的分子标记辅助育种,并为茎瘤芥耐抽薹品种的选育提供理论依据。The early bolting of tumorous stem mustard caused tremendous loss to mustard production, which became one of the major factors hampering the rapid and healthy development of the mustard industry. In the present study, bulked segregant analysis （BSA） was used to identify the SSR markers related to bolting genes in the F2 generation of an early bolting cultivar ＇ 92 ＇ and a late bolting cultivar ＇ 203＇ of tumorous stem mustard. 600 pairs of Brassica genus SSR primers were used to identify the polymorphism, and one SSR marker Ol12-D09 was found in the early bolting gene pool, with a close genetic distance of 10.9 cM calculated by the kosambi function. The SSR marker identified in the present study can be used for molecular marker-assisted breeding in the early bolting of tuber mustard. The findings can also provide theoretical foundations for the breeding of bolting-resistant tuber mustard.

关 键 词：茎瘤芥 抽薹 集团分离分析法 SSR标记 

分 类 号：S639.9[农业科学—蔬菜学]