中黑盲蝽胚胎发育的显微及切片观察  

Morphological observation of embryonic development of Adelphocoris suturalis by microscope and paraffin section

作　　者：田彩红[1] 封洪强[1] 王瑛[1] 许鹏[1,2] 李国平[1] 黄建荣[1] 封洪云[1] 尹新明[2] Tian Caihong Feng Hongqiang Wang Ying Xu Peng Li Guoping Huang Jianrong Feng Hongyun Yin Xinming(Henan Key Laboratory of Crop Pest Control, Key Laboratory of Crop Integrated Pest Management （IPM） in Southern Part of Northern China, Ministry of Agriculture, International Joint Research Laboratory for Crop Protection of Henan, Biological Pesticides Engineering Research Center of Henan Province, Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China College of Plant Protection, Henan Agricultural University, Zhengzhou 450002, China)

机构地区：[1]河南省农作物病虫害防治重点实验、农业部华北南部作物有害生物综合治理重点实验室、河南省作物保护国际联合实验室、河南省生物农药工程研究中心、河南省农业科学院植物保护研究所,郑州450002 [2]河南农业大学植物保护学院,郑州450002

出　　处：《植物保护》2017年第1期178-181,共4页Plant Protection

基　　金：公益性行业(农业)科研专项(201103012-7);国家自然科学基金(31101449)

摘　　要：以滞育卵越冬是中黑盲蝽生活史的重要组成部分,为明确滞育卵胚胎发育的停滞时期,对中黑盲蝽的胚胎发育过程进行了显微观察,并将胚胎发育停滞期的滞育卵做了石蜡切片。温度(26±1)℃,相对湿度70%±10%,光周期L∥D=14h∥10h条件下,滞育卵在产卵后48h停止发育,而非滞育卵则继续发育,在产卵后第9天孵出若虫。通过测定中黑盲蝽胚胎发育不同时期的卵重发现,非滞育卵随着胚胎发育卵重持续增加,滞育卵在发育48h后卵重不再增加。Adelphocoris suturalis overwinters with diapause eggs laid in the stems of cotton, tissue of weed, fruit trees and alfalfa hosts adjacent to the cotton fields, which is the most critical link of its life history. To identify the period of the embryo development stagnation in diapause eggs, we performed a morphological observation using microscope and paraffin section. Eggs, nymphs and adults were reared on green beans at （26 ± 1 ）℃, 70% ± 10% relative humidity, and a photoperiod cycle of 14 h light/10 h dark. The embryo stopped growing within 48 hours after oviposition in diapause eggs. While in the non-diapause eggs, the embryo kept developing with cells differentiation, 9 days after oviposition, the nymphs were hatched. The weight of non-diapause eggs continued to increase with the development of embryo. However, the weight of diapause eggs maintained stability after the formation of diapause.

关 键 词：中黑盲蝽 胚胎发育 滞育卵 石蜡切片 卵重 

分 类 号：S433[农业科学—农业昆虫与害虫防治]