Correlation between receptor-interacting protein 140 expression and directed differentiation of human embryonic stem cells into neural stem cells  

作　　者：Zhu-ran Zhao Wei-dong Yu Cheng Shi Rong Liang Xi Chen Xiao Feng Xue Zhang Qing Mu Huan Shen Jing-zhu Guo 

机构地区：[1]Department of Pediatrics,Peking University People's Hospital,Beijing,China [2]Institute of Clinical Molecular Biology,Peking University People's Hospital,Beijing,China [3]Department of Obstetrics and Gynecology,Peking University People's Hospital,Beijing,China [4]Department of Pediatrics,Peking University International Hospital,Beijing,China

出　　处：《Neural Regeneration Research》2017年第1期118-124,共7页中国神经再生研究（英文版）

基　　金：supported by the National Natural Science Foundation of China,No.31340024

摘　　要：Overexpression of receptor-interacting protein 140（RIP140） promotes neuronal differentiation of N2 a cells via extracellular regulated kinase 1/2（ERK1/2） signaling.However,involvement of RIP140 in human neural differentiation remains unclear.We found both RIP140 and ERK1/2 expression increased during neural differentiation of H1 human embryonic stem cells.Moreover,RIP140 negatively correlated with stem cell markers Oct4 and Sox2 during early stages of neural differentiation,and positively correlated with the neural stem cell marker Nestin during later stages.Thus,ERK1/2 signaling may provide the molecular mechanism by which RIP140 takes part in neural differentiation to eventually affect the number of neurons produced.Overexpression of receptor-interacting protein 140（RIP140） promotes neuronal differentiation of N2 a cells via extracellular regulated kinase 1/2（ERK1/2） signaling.However,involvement of RIP140 in human neural differentiation remains unclear.We found both RIP140 and ERK1/2 expression increased during neural differentiation of H1 human embryonic stem cells.Moreover,RIP140 negatively correlated with stem cell markers Oct4 and Sox2 during early stages of neural differentiation,and positively correlated with the neural stem cell marker Nestin during later stages.Thus,ERK1/2 signaling may provide the molecular mechanism by which RIP140 takes part in neural differentiation to eventually affect the number of neurons produced.

关 键 词：nerve regeneration receptor-interacting protein 140 neural stem cells human embryonic stem cells directed differentiation Oct4 Sox2 Nestin extracellular regulated kinase 1/2 signaling pathway neural regeneration 

分 类 号：R338[医药卫生—人体生理学]