清肝化痰活血方对非酒精性脂肪性肝炎模型大鼠水通道蛋白7、9表达的影响  

Effects of Qinggan Huatan Huoxue Decoction on the expressions of aquaglyceroporin-7,9 in nonalcoholic steatohepatitis rats

作　　者：李英[1] 王晓素[1] 孔婧[1] 周莉[1] 陈宇华[1] LI Ying WANG Xiao-su KONG Jing ZHOU Li CHEN Yu-hua(Department of Gastroenterology, Yueyang Hospital of Integrated Chinese ＆ Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai,20043)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院消化科,上海200437

出　　处：《上海中医药杂志》2017年第2期76-80,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市教委预算内项目(2012JW54)

摘　　要：目的观察清肝化痰活血方对非酒精性脂肪性肝炎模型大鼠水通道蛋白7、9(AQP-7、9)表达的影响。方法采用高脂饲料喂养复制模型大鼠,第8周分别处死正常组大鼠5只和模型组大鼠10只,模型组剩余大鼠再分为高脂饲料组、易善复组和清肝化痰活血方组,给药5周后,处死大鼠,留取血清、肝组织标本,检测血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、三酰甘油(TG)、胆固醇(TC)及肝组织中AQP-7、9的表达;另肝组织经HE染色光镜下观察肝组织病理学变化。结果第8周时,模型组大鼠脂肪变性程度和炎症活动度计分与正常组比较有显著性差异(P<0.01)。第13周时,高脂饲料组大鼠肝细胞脂肪变性和炎症活动度计分与正常组、西药组和中药组相比有显著性差异(P<0.05,P<0.01),肝组织中AQP-7、AQP-9 mRNA的含量与正常组、西药组和中药组相比差异有统计学意义(P<0.01);清肝化痰活血方组大鼠肝组织脂肪变性程度和炎症活动度计分与高脂饲料组比较差异有统计学意义(P<0.05),血清AST、ALT、TG、TC的含量与高脂饲料组比较明显下降(P<0.01),肝组织中AQP-7含量较高脂饲料组明显上升(P<0.01),AQP-9含量较高脂饲料组明显下降(P<0.01)。结论 AQP-7、9与非酒精性脂肪性肝炎的发病有关,清肝化痰活血方可能通过调节AQP-7、9的表达进而改善肝细胞脂质代谢起到防治非酒精性脂肪性肝炎的作用。Objective To observe the effects of Qinggan Huatan Huoxue Decoction on the expressions of aquaglyceroporin-7（ AQP-7） and-9（ AQP-9） in nonalcoholic steatohepatitis（ NASH） rats. Methods Rats model was copied by the feeding of high fat diet. On the 8th week,5 rats in the normal group and 10 rats in the model group were sacrificed respectively. Then the remaining rats in the model group were divided into the high fat diet group,Yishanfu Tablet group and Qinggan Huatan Huoxue Decoction group. After administration for 5 weeks,the rats were sacrificed and the serum and liver tissue samples were collected. The serum levels of alanine aminotransferase（ ALT）,aspartate aminotransferase（ AST）,triacylglycerol（ TG）,cholesterol（ TC） and the expression of AQP-7,9 in liver tissue were detected. The pathological changes of liver tissues were observed by HE staining under light microscope. Results On 8th week,there were significant differences on the degree of steatosis and score of inflammatory activity between the model group and normal group（ P〈 0. 01）. On 13 th week,there were significant differences on the degree of steatosis and score of inflammatory activity between the high fat diet group and normal group,western medicine group,Chinese medicine group（ P 〈0. 05,P 〈0. 01）. There were significant differences on the levels of AQP-7 and AQP-9 mRNA between the high fat diet group and normal group,western medicine group,Chinese medicine group（ P 〈0. 01）. There were statistically significant differences on the degree of steatosis and score of inflammatory activity between the high fat diet group and Qinggan Huatan Huoxue Decoction group（ P〈 0. 05）. Compared with the high fat diet group,the contents of AST,ALT,TG,TC in the Qinggan Huatan Huoxue Decoction group were significantly decreased（ P 〈0. 01）,the level of AQP-7 in liver tissue was significantly increased（ P 〈0. 01） and the level of AQP-9 was significantly decreased（ P 〈0. 01）. Conclusion AQP-7,9 are assoc

关 键 词：非酒精性脂肪性肝炎 清肝化痰活血方 水通道蛋白7、9 

分 类 号：R285.5[医药卫生—中药学] R-332[医药卫生—中医学]