3种植物浸液对白纹伊蚊产卵的影响研究  

Oviposition responses of Aedes albopictus to three plant infusions

作　　者：李凯利[1,2] 高景鹏[2] 曹洁[1] 马雅军[2] 

机构地区：[1]第二军医大学基础医学实验教学中心,上海200433 [2]第二军医大学热带医学与公共卫生学系热带传染病学教研室,上海200433

出　　处：《中国媒介生物学及控制杂志》2017年第1期4-8,共5页Chinese Journal of Vector Biology and Control

基　　金：国家自然科学基金(81371848)~~

摘　　要：目的筛选具有诱集白纹伊蚊产卵活性的植物浸液,并分析其化学成分。方法选择稻草、竹叶和狗牙根浸泡不同时间,在实验室和现场进行白纹伊蚊诱集产卵实验,利用气相色谱-质谱联用仪(GC-MS)分析植物浸液中的挥发性化合物。结果实验室稻草和竹叶浸液均有诱集白纹伊蚊产卵的活性,狗牙根浸液的诱集活性较差。稻草及竹叶浸液的平均诱卵率分别为63.20%~80.20%和47.51%~86.34%,平均产卵活跃指数(OAI)分别为0.26~0.60和-0.05~0.76;稻草浸泡30~70 d,竹叶浸泡50~70d具有明显的诱集活性,浸泡时间越长,诱集效果越好。应用浸泡≥50d的浸液进行现场实验,稻草及竹叶浸液的平均诱卵率分别为70.99%~96.17%和69.52%~89.51%;平均OAI分别为0.42~0.93和0.39~0.79。GC-MS鉴定不同浸泡时间稻草浸液中的化合物共108种,竹叶浸液有97种,其中相对含量>3%的化学物共17种,2,4-二苯酚(C_(14)H_(22)O)和苯甲醛(C_9H_(17)O)在所有样本中均存在,且含量较高。结论稻草和竹叶浸泡一定时间后,其浸液具有明显的诱集白纹伊蚊产卵活性,主要化学成分为2,4-二苯酚和苯甲醛。Objective To evaluate the effect of different plant infusions on the oviposition behavior of female Aedes albopictus and analyze the chemical composition. Methods The plant infusions were made by soaking leaves of Oryza sativa, Bambusaceae and Cynodon dactylon in sterile water for different times. The oviposition preference of gravid Ae. albopictus to plant infusions was tested in laboratory and field. Gas chromatography-mass spectrometer （GC-MS） was used to detect the chemical compounds of the plant infusions. Results It was different oviposition response of Ae. albopictus laboratory colony to O. sativa, Bambusaceae and C. dactylon infusions. The average spawning rate and oviposition activity index （OAI） were 63.20%-80.20% and 0.26-0.60 in O. sativa infusion; 47.51%-86.34% and -0.05-0.76 in Bambusaceae infusion, respectively. There was no oviposition preference in C. dactylon infusions. The effect of oviposition preference was significant from 30 d （O. sativa） and 50 d （Bambusaceae） to 70 d of soaking time. The trend was that the longer soaking time, the better of attractancy effects. The infusions of O. sativa and Bambusaeeae after 50 d soaking time were used to test in field. The average spawning rate and its OAI were 70.99%-96.17% and 69.52%-89.51%, 0.42-0.93 and 0.39-0.79 in O. sativa infusions and Bambusaceae infusions. The GC-MS results showed that O. sativa infusion contained 108 chemical compounds and Bambusaceae infusions with 97 compounds. Of them, there were 17 compounds whose relative percentage more than 3%. The 2,4-his （1, 1-dimethylethyl）-phenol and Benzaldehyde were included in all samples and had higher relative content. Conclusion The infusions of O. sativa and Bambusaceae leaf had significant effect on oviposition attractancy after soaking for certain period time, and the major chemical compounds were 2,4-bis （1,1-dimethylethyl）- phenol and Benzaldehyde.

关 键 词：稻草浸液 竹叶浸液 产卵 白纹伊蚊 气相色谱-质谱联用仪 

分 类 号：R384.1[医药卫生—医学寄生虫学] S482.892[医药卫生—基础医学]