Cl^-通道抑制剂对高氯条件下烤烟叶片超微结构和生理指标的影响  

Effects of Chloride Channel Inhibitors on Ultrastructure and Some Physiological Indexes under the High Chloride in Flue-Cured Tobacco Leaves

作　　者：商静[1] 许嘉阳 王小东[3] 苏良天 黄五星[1] 许自成[1] SHANG Jing XU Jiayang WANG Xiaodong SU Liangtian HUANG Wuxing XU Zicheng(College of Tobacco Science, Henan Agricultural University, Zhengzhou 450002 Agronomy and Biotechnology College, China Agricultural University, Beijing 100193 College of Agriculture, Henan University of Science and Technology, Henan Luoyang 471003, China)

机构地区：[1]河南农业大学烟草学院,郑州450002 [2]中国农业大学农学与生物技术学院,北京100193 [3]河南科技大学农学院,河南洛阳471003

出　　处：《中国农业科技导报》2017年第2期20-27,共8页Journal of Agricultural Science and Technology

基　　金：中国烟草总公司河南省公司重点科技攻关项目(HYKJ201405)资助

摘　　要：通过土培盆栽试验,研究了高氯条件下Zn^(2+)、NFA(尼氟灭酸)、9-AC(蒽-9-羧酸)3种Cl-通道抑制剂对烤烟叶片超微结构和相关生理指标变化的影响。结果表明:与对照相比,外施抑制剂可显著缓解氯含量较高的土壤对烤烟叶片的伤害作用,其中9-AC缓解作用最强,NFA次之,Zn2+作用较弱。施用抑制剂处理的烤烟叶片,其类囊体结构较发达,片层较多,垛叠能力强;抑制剂对烤烟生物量的积累有促进作用,其中地上部的干物质重提高显著;与对照相比,烤烟叶片MDA含量明显下降,在一定程度上缓解了膜脂的过氧化,同时,施用抑制剂有利于SOD、POD、CAT等抗氧化保护酶维持较高的活性。A pot experiment was conducted to investigate the effects of three kinds of chloride channel inhibitors 200 μmol/L Zn^2＋, 200 μmol/L NFA and 200 μmol/L 9-AC on ultrastructure and some physiological indexes in fluecured tobacco leaves under the high chloride. The results showed that, compared with CK, treatments with inhibitors obviously alleviated the injuries of the high chloride upon the flue-cured tobacco leaves. And the effect of the 3 inhibitors on alleviating injuries was in the order of 9-AC, NFA, Zn^2＋. Furthermore, inhibitors treatments resulted in the thylakoids well developed, which had more lamellae and strong stacking capability. Different inhibitors treatments had a significant effect on accumulation of biomass and dry matter, especially for above-ground. In addition, inhibitors treatments obviously decreased the content of MDA, which represented the production of lipid peroxide. With the supply of inhibitors were favorable to maintaining the activities of SOD, POD and CAT at high level in fluecured tobacco leaves.

关 键 词：Cl^-通道抑制剂 烤烟 超微结构 生理特性 

分 类 号：S572[农业科学—烟草工业]