非对称性二甲基精氨酸对豚鼠左心室流出道自律细胞电活动的影响  

Influence of ADMA in electrical activity of pacemaker cells in guinea-pig left ventricular outflow tract

作　　者：赵兰平[1] 薛淑芳[1] 陈彦静[2] 王雪芳[1] 陈立锋[1] 黄嘉诚[1] 

机构地区：[1]河北北方学院生理学教研室,河北张家口075000 [2]中国中医科学院中医基础理论研究所,北京100700

出　　处：《吉林大学学报（医学版）》2017年第1期6-10,共5页Journal of Jilin University：Medicine Edition

基　　金：河北省教育厅高等学校科学技术研究重点项目资助课题(ZD20131054);北方学院创新人才培育项目资助课题(CXRC1324)

摘　　要：目的:研究内源性一氧化氮合酶(NOS)抑制物非对称性二甲基精氨酸(ADMA)对豚鼠左心室流出道自律细胞电活动的影响,探讨ADMA在源于心室流出道心律失常发生发展中的作用。方法:制备豚鼠离体左心室流出道标本,用氧饱和的改良Locke液进行恒温、恒速灌流,采用标准玻璃微电极细胞内电位记录技术记录豚鼠离体左心室流出道部位的自发慢反应电位(sAP),观测该电位对ADMA的浓度依赖性效应,ADMA分为3个浓度组(1、10和100μmol·L^(-1));用NO供体硝普钠(SNP)灌流预处理标本,分为对照组、SNP(10μmol·L^(-1))组和SNP+ADMA组,记录并分析SNP预处理对ADMA所致左心室流出道电生理效应的影响。结果:与对照组比较,1μmol·L^(-1) ADMA组sAP各项指标均无明显变化(P>0.05),10和100μmol·L^(-1) ADMA组4相自动去极速度(VDD)、自发放电频率(RPF)和0相最大除极速度(Vmax)明显减慢(P<0.05),复极50%和90%时间(APD50和APD90)明显延长(P<0.05),100μmol·L^(-1) ADMA组动作电位幅度(APA)明显减小(P<0.05);与1μmol·L^(-1) ADMA组比较,10μmol·L^(-1) ADMA组RPF、APD90及100μmol·L^(-1) ADMA组RPF、Vmax和APD50差异有统计学意义(P<0.05);与10μmol·L^(-1) ADMA组比较,100μmol·L^(-1) ADMA组APA明显减小(P<0.05)。10μmol·L^(-1) SNP预处理标本10 min可逆转ADMA降低左心室流出道自发放电活动的效应,与对照组比较,VDD和RPF仍明显加快(P<0.05),Vmax和APA有恢复至对照组水平的趋势(P>0.05),APD90明显缩短(P<0.05)。结论:内源性NOS抑制物ADMA浓度依赖性降低左心室流出道自律细胞的自发放电活动,NO供体SNP预处理可逆转上述效应。Objective： To study the influence of endogenous nitric oxide synthase （NOS） inhibitor asymmetric dimethylarginine （ADMA） in the electrical activity of pacemaker cells in the guinea-pig left ventricular outflow tract, and to explore the effect of ADMA on the occurrence and development of ventricular outflow tract arrhythmias. Methods： The isolated guinea-pig left ventricular outflow tract tissue was perfused with fully oxygenated Locke solution under constant temperature and constant flow velocity. The spontaneous slow action potentials （sAP） of guinea-pig left ventricular tract were recorded by conventional intracellular microelectrode technique. The effects of NOS inhibitor ADMA （1, 10 and 100μmol ·L-1） on the spontaneous sAP were studied. And the effects of ADMA under the condition of pretreatment of sodium nitroprusside （SNP） were also explored （control group, 10μmol · L-1 SNP group, and SNPq-ADMA group）. FtesuLts Compared with control group, the different indexes of sAP in μmol·L-1 ADMA group had no significant changes （P〈0.05）. Compared with control group, the velocity of diastolic depolarization （VDD）, the rate of pacemaker firing （RPF） and the maximal rate of depolarization （Vmax） in 10 and 100μmol · L 1 ADMA groups were siginificantly decreased （P〈0.05）, and the 50% and 90% of duration of action potential （APDs0 and APDg0） were markedly increased （P〈0.05）. Compared with 1 μmol · L-1ADMA group, the RPF in 10μmol· L-1 ADMA group was siginificantly decreased （P〈0.05） and the APDg0 was markedly increased （P〈0.05）; compared with 1 μmol·L-1 ADMA group, the RPE, V and APDs0 had significant changes （P0.05）. Compared with 10 μmol · L-1 ADMA group, the APA in 100 μmol ·L 1 ADMA group was significantly decreased （P%0.05）. After pretreatment of 10 〉mol·L-1 SNP for 10 min, followed by 10 μmol · L-1 SNP and 10 μmol · L-1 ADMA, the inhibitory effects of spontaneous activity induced by ADMA could be reversed compare

关 键 词：非对称性二甲基精氨酸 左心室流出道 自发慢反应电位 

分 类 号：Q463[生物学—生理学] R331.3[医药卫生—人体生理学]