运动对RBP4诱导的脂代谢异常鼠肝脏ACC1表达和脂肪合成的影响  

Effects of Exercise on ACC1 Expression and Fat Synthesis of Liver in RBP4-induced Dyslipidemia Mice

作　　者：张明军[1] 魏丽香[1] ZHANG Ming-jun WEI Li-xiang(Department of Physical Education, Huizhou University, Huizhou 516007, China)

机构地区：[1]惠州学院体育系,广东惠州516007

出　　处：《河北体育学院学报》2017年第1期54-59,共6页Journal of Hebei Sport University

基　　金：福建省自然科学基金项目(2011J01151);广东省哲学社会科学规划项目(GD15XTY10)

摘　　要：目的:研究运动对RBP4诱导的脂代谢异常鼠肝脏ACC1表达和脂肪合成的影响。方法:重组人RBP4注射建立脂代谢异常鼠模型。设一次性运动组(RBP4 One-time exercise group,ROE组)、8周有氧运动组(RBP4aerobic exercise group,RAE组)和安静对照组(RBP4control group,RC组)。结果:ROE组血清RBP4水平与RC组相比显著降低(P<0.05),血清TG和肝脏TG含量无显著改变(P>0.05)。RAE组血清RBP4、TG和肝脏TG含量与RC组和ROE组相比均显著降低(P<0.01)。与RC组相比,ROE组和RAE组肝脏ACC1mRNA表达均显著降低(P<0.01)。与RC组相比,ROE组ACC1蛋白表达无显著改变(P>0.05)。与RC组和ROE组相比,RAE组ACC1蛋白表达均显著降低(P<0.01)。结论:高RBP4显著增加小鼠肝脏ACC1基因和蛋白表达增加,促进肝脏脂肪合成。8周有氧运动抑制了RBP4在肝脏的脂肪合成促进作用,改善脂代谢,机制涉及降低RBP4水平、减少肝脏ACC1基因和蛋白表达以及TG的合成。Objective：To study effects of exercise on ACC1 expression and fat synthesis of liver in RBP4-induced dyslipidemia Mice.Method：RBP4-induced dyslipidemia mice model established by RBP4 intraperitoneal injection.Model rats are divided in RBP4 One-time exercise group（ROE,8-weeks RBP4 aerobic exercise group（RAE）,and RBP4 control group（RC）.Results：Serum RBP4 decreases more in group ROE than group RC（P〈0.05）.There are no significant differences in serum TG and liver TG between group ROE and group RC（P〉0.05）.Serum RBP4,TG,and liver TG decreases more in group RAE than group RC and group ROE respectively（P〈0.01）.Liver ACC1 mRNA expression decreases more in group ROE and group RAE than group RC（P〈0.01）.There are no significant differences in ACC1 protein expression between group RC and group ROE（P〉0.05）.Liver ACC1 protein expression decreases more in group RAE than group RC and group ROE respectively（P〈0.01）.Conclusions：RBP4increases dramatically ACC1 mRNA expression in mice liver,and facilitates liver fat synthesis.8-weeks aerobic exercise could suppress liver fat synthesis,and improve lipid metabolism.The mechanism involves in lowering RBP4,reducing liver ACC1 expression,and TG synthesis after 8-weeks aerobic exercise.

关 键 词：运动 视黄醇结合蛋白4 乙酰辅酶A羧化酶1 脂肪合成 脂肪代谢 

分 类 号：G804[文化科学—运动人体科学]