木犀草素对东莨菪碱所致大鼠学习记忆障碍的改善作用  

Ameliorative Effects of Luteolin on Learning and Memory Impairment Induced by Scopolamine in Rats

作　　者：张永亮[1] 陈海龙[2] 王婷梅[1] 王艳利[1] 冀国华[2] 李莹辉[1,2] 曲丽娜[2] ZHANG Yong-liang CHEN Hai-long WANG Ting-mei WANG Yan-li JI Guo-hua LI Ying-hui QU Li-na(School of Life Sciences, Northwestern Polytechnical University, Xi＇an 710072, China State Key Lab of Space Medicine Fundamental and Application, China Astronaut Research and Training Center ,Beijing 100094,China)

机构地区：[1]西北工业大学生命学院,陕西西安710072 [2]中国航天员科研训练中心航天医学基础与应用国家重点实验室,北京100094

出　　处：《化学与生物工程》2017年第2期33-37,49,共6页Chemistry & Bioengineering

基　　金：国家科技重大专项(2012ZX09J12201);全军医药卫生"十二五"科研项目(BWS11J052);航天医学基础与应用国家重点实验室自主研究项目(SMFA15B01)

摘　　要：研究了木犀草素对东莨菪碱所致大鼠学习记忆障碍的改善作用。将SD大鼠随机分为6组:正常对照组、模型组、多奈哌齐组和不同剂量(30mg·kg^(-1)、60mg·kg^(-1)、120mg·kg^(-1))木犀草素组,腹腔注射东莨菪碱建立大鼠学习记忆障碍模型,采用Morris水迷宫实验和避暗实验测试大鼠学习记忆能力,同时测定大鼠海马组织超氧化物歧化酶(SOD)活性、乙酰胆碱酯酶(AchE)活性及丙二醛(MDA)含量。结果表明:与模型组比较,木犀草素(120mg·kg^(-1))处理可显著缩短大鼠在Morris水迷宫实验中的逃避潜伏期(P<0.05或P<0.01),增加大鼠穿台次数、目的象限时间比和游程比(P<0.05),延长避暗实验中大鼠的入暗潜伏期(P<0.01);同时木犀草素(120mg·kg^(-1))处理可提高大鼠海马组织SOD活性(P<0.01),降低AchE活性(P<0.05)及MDA含量。木犀草素可通过抑制海马区氧化损伤和增强胆碱能功能改善东莨菪碱所致大鼠学习记忆障碍。Ameliorative effects of luteolin on learning and memory impairment induced by scopolamine in rats were studied.SD rats were randomly divided into six groups：control group,model group,donepezil group and luteolin groups（30mg·kg-1,60mg·kg-1,120mg·kg-1）.Scopolamine was intraperitoneally injected to establish learning and memory impairment model in rats.Morris water maze test and step-through test were applied to investigate learning and memory ability of rats.Meanwhile,SOD activity,AchE activity and MDA content in rat hippocampus tissues were also detected.Results indicated that,compared with the model group,luteolin（120 mg·kg-1）treatment significantly decreased the escaping latency（P〈0.05 or P〈0.01）,increased crossing times and the percentage of time in the platform quadrant of rats in Morris water maze test（P〈0.05）,extended the dark latency in step-through test（P〈0.01）.On the other hand,luteolin（120mg·kg-1）treatmentenhanced SOD activity in rat hippocampus tissues（P〈0.01）,while AchE activity（P〈0.05） and MDA content were depressed.Through inhibiting oxidative damage in hippocampus tissues and enhancing cholinergic function,luteolin treatment could significantly ameliorate the learning and memory impairment induced by scopolamine in rats.

关 键 词：木犀草素 东莨菪碱 学习记忆 改善作用 

分 类 号：R285.5[医药卫生—中药学]