N,N-二丙基异鲁米诺的合成及其光谱性质  

Synthesis and Spectral Properties of N,N-Dipropylisoluminol

作　　者：伍斌[1] 沈娟[1] 曹敏[2] 冯志明[1] WU Bin SHEN Juan CAO Min FENG Zhi-ming(Key Laboratory of Chemical Biology ＆ Traditional Chinese Medicine Research of Ministry of Education, Hunan Normal University ,Changsha 410081 ,China School of Economics ~ Management ,Changsha University of Science ＆ Technology ,Changsha 410076,China)

机构地区：[1]湖南师范大学化学生物学及中药分析省部共建教育部重点实验室,湖南长沙410081 [2]长沙理工大学经济与管理学院,湖南长沙410076

出　　处：《化学与生物工程》2017年第2期42-44,共3页Chemistry & Bioengineering

摘　　要：合成了异鲁米诺衍生物N,N-二丙基异鲁米诺,对其结构和光谱性质进行了表征与研究。结果表明,pH=2~4时,N,N-二丙基异鲁米诺有较强的荧光,且随着pH值的增大荧光强度先增强后减弱,在pH=3时,荧光强度达到最强;pH=5~6时,荧光强度随着pH值的增大逐渐减弱;pH=7~12时,荧光强度基本稳定。在N,N-二丙基异鲁米诺的浓度为0~50μmol·L^(-1)时,荧光强度随浓度的增大而增强,线性拟合的标准曲线方程为y=4.5371+7.9074x,相关系数R2为0.9972。紫外吸收光谱表明,N,N-二丙基异鲁米诺在296nm处有很强的π→π*跃迁和苯环的重叠引起的B带吸收峰,在200~240nm处有羰基与苯环双键共轭引起的较弱的K带吸收峰。Isoluminol derivative N,N-dipropylisoluminol was synthesized,its structure was characterized,and its spectral properties were studied.When pH=24,N,N-dipropylisoluminol had strong fluorescence,and the fluorescence intensity increased firstly and then weakened with the increasing of pH value,when pH=3,the fluorescence intensity was the strongest;when pH=56,the fluorescence intensity gradually weakened with the increasing of pH value;when pH=712,the fluorescence intensity was basically stable.When the concentration of N,N-dipropylisoluminol was 050μmol·L-1,the fluorescence intensity increased with the increasing of the concentration.The standard curve equation of linear fitting was y=4.5371＋7.9074 x,and the correlation coefficient R2 was 0.9972.Ultraviolet absorption spectrum showed that,N,N-dipropylisoluminol had strongπ→π＊ transition and B band absorption peak caused by overlapping benzene ring at 296 nm,and conjugate weak K band absorption peak between carbonyl and the double bond of benzene ring at 200240nm.

关 键 词：N N-二丙基异鲁米诺 紫外吸收光谱 荧光强度 

分 类 号：TQ422[化学工程] O621.22[理学—有机化学]