高效液相色谱法测定菊芋叶和向日葵叶中绿原酸  

Determination of Contents of Chlorogenic Acid in Helianthus tuberosus L.Leaves and Helianthus annuus L.Leaves by HPLC

作　　者：苗芹 叶明国[3] 刘苏静[1] 衣悦涛[1] 夏传海[4] MIAO Qin YE Ming-guo LIU Su-jing YI Yue-tao XIA Chuan-hai(Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences ,Yantai 264003, China Yantai Center for Integrated Technology Transfer Center,Chinese Academy of Sciences , Yantai 264003, China College of Resources and Environmental Engineering ,Ludong University ,Yantai 264025, China University of Chinese Academy of Sciences ,Beijing 100049 ,China)

机构地区：[1]中国科学院烟台海岸带研究所,山东烟台264003 [2]中国科学院大学,北京100049 [3]中国科学院山东综合技术转化中心烟台中心,山东烟台264003 [4]鲁东大学资源与环境工程学院,山东烟台264025

出　　处：《化学与生物工程》2017年第2期63-67,共5页Chemistry & Bioengineering

基　　金：十二五"国家科技支撑计划资助项目(2011BAC02B04);山东省科技重大专项(2015ZDJS03002);山东省高等学校优势学科人才团队培育计划"蓝黄两区滨海资源与环境团队

摘　　要：采用HPLC法测定菊芋叶和向日葵叶中绿原酸含量,并对收获期菊芋叶和向日葵叶中绿原酸的积累分布进行研究。色谱条件为:ZORBAX Extend-C_(18)(4.6mm×250mm,5μm),柱温30℃,检测波长327nm,进样量10μL,流速1.0mL·min^(-1),流动相为乙腈-水(含体积分数为0.05%的甲酸),梯度洗脱。提取条件:甲醇-水(70∶30,体积比)回流浸提,料液比1∶25(g∶mL),提取时间1.5h,提取温度60℃。结果表明,菊芋叶和向日葵叶中的绿原酸在收获期积累分布差异显著,菊芋叶中绿原酸含量在10月下旬最高,为4.20%;向日葵叶中绿原酸含量在8月下旬最高,为0.47%。因此,10月下旬的菊芋叶可以作为提取绿原酸的植物原材料。The contents of chlorogenic acid（3-CQA）in Helianthus tuberosus L.leaves and Helianthusann uusL.leaves were determinated by HPLC.The content accumulated distributions of 3-CQA in Helianthus tuberosus L.leaves and Helianthus annuus L.leaves at harvest time were investigated.The chromatographic conditions were as follows：a ZORBAX Extend-C18column（4.6 mm×250 mm,5μm）,column temperature of 30 ℃,detection wavelength of 327 nm,injection volume of 10μL,flow rate of 1.0mL·min-1,gradient mobile phase of acetonitrile-water（containing 0.05%formic acid）.The optimal extraction conditions were as follows：solvent refluxing extraction with methanol-water（70∶30,volume fraction）,material-liquid ratio of 1∶25（g∶mL）,extraction time of 1.5h,extraction temperature of 60℃.The results demonstrated that the content accumulated distributions of 3-CQA in Helianthus tuberosus L.leaves and HelianthusannuusL.leaves were significant different at harvest time.The content of 3-CQA in Helianthus tuberosus L.leaves reached the highest level（4.20%）in late October.The content of 3-CQA in Helianthus annuus L.leaves reached the highest level（0.47%）in late August.Thus,Helianthus tuberosus L.leaves in late October could be used as the raw material for extracting chlorogenic acid.

关 键 词：绿原酸 菊科植物 菊芋叶 向日葵叶 高效液相色谱 

分 类 号：O657.72[理学—分析化学] R284.2[理学—化学]