儿童过敏性紫癜血中标志物的检测及临床意义  被引量:20

Clinical significance of serum markers in Henoch-Schonlein purpura in children

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作  者:段文冰[1] 鞠瑛[1] 沈亚娟[1] 刘义庆[1] 张璐璐[1] 陈兰兰[1] 张培莉[1] 张炳昌[1] 

机构地区:[1]山东大学附属省立医院检验医学部,济南250000

出  处:《中国医药》2017年第2期285-289,共5页China Medicine

基  金:山东省自然科学基金(ZR2016HM52);山东省科学技术发展计划(2014GGH218041);山东省临床重点专科建设项目(鲁卫医字2013-26)

摘  要:目的探讨临床实验室常用检测指标对过敏性紫癜(HSP)患儿的临床应用价值。方法选取2015年1-7月于山东大学附属省立医院住院的HSP患儿108例(HSP组),按有无肾脏损害分为紫癜性肾炎组(HSPN组,70例)和非紫癜性肾炎组(NHSPN组,38例),并同时选取45名健康体检儿童作为对照组。分别应用半导体激光核酸荧光染色流式细胞术检测血常规,流式细胞仪免疫荧光法检测T淋巴细胞亚群、自然杀伤细胞、B淋巴细胞,散射比浊法检测血清免疫球蛋白(Ig)、血清补体,免疫比浊法检测D-二聚体,终点法检测24h尿蛋白。比较HSP组与对照组、HSPN组与NHSPN组白细胞计数、淋巴细胞百分比、中性粒细胞百分比、血小板计数、CD3+、CD3+CD4+、CD3+CD8+、CD3-CD19+、CD16+CD56+、IgG、IgA、IgM、IgE、补体C3、补体C4、D-二聚体和24h尿蛋白。结果HSP组白细胞计数、淋巴细胞百分比、血小板计数、CD3+CD8+、CD3-CD19+、IgA、IgE、补体C3、D-二聚体、24h尿蛋白均明显高于对照组[(8.7±3.5)×10^9/L比(5.9±1.6)×10^9/L、(39±16)%比(35±6)%、(275±79)×10^9/L比(219±65)×10^9/L、(30±6)%比(24±4)%、(19±10)%比(13±3)%、(2.5±1.1)g/L比(1.4±0.4)g/L、(170±168)kIU/L比(46±34)kIU/L、(1.30±0.32)g/L比(1.23±0.24)g/L、(1840±1720)μg/L比(260±120)μg/L、0.10(0.04,0.48)g比0.06(0.04,0.09)g],CD3+、CD3+CD4+、CD16+CD56+、IgG均明显低于对照组[(67±9)%比(81±5)%、(32.1±1.7)%比(52.6±3.3)%、(9±3)%比(15±5)%、(8.0±3.0)g/L比(8.7±1.3)g/L],差异均有统计学意义(均P〈0.05)。HSPN组IgA、补体C3、D-二聚体、24h尿蛋白均明显高于NHSPN组,IgE和中性粒细胞百分比明显低于NHSPN组,差异均有统计�Objective To analyze the clinical significance of serum markers in Henoch-Schonlein purpura (HSP) in children. Methods From January to July 2015, 108 children with HSP in Shandong Provincial Hospital Affiliated to Shandong University were enrolled ( HSP group) ; the children were divided into Henoch-Schonlein purpura nephritis(HSPN) group with 70 cases and non-HSPN(NHSPN) group with 38 cases; 45 healthy children were enrolled as control group. Blood routine was tested by semiconductor laser nucleic acid fluorescence staining flow cytometry; T lymphocyte subsets, natural killer cell and B lymphocyte were tested by flow cytometry immunofluorescence; serum immunoglobulin (Ig) and serum complement were tested by scattering turbidimetry; D-dimer was tested by immune turbidimetry ; 24 h urine protein was tested by end-point method. Leukocyte count,lymphocyte percentage, neutrophils percentage, platelet count, CD3+, CD3+CD4+, CD3+CD8+, CD3-CD19+, CD16+CD56+, IgG, IgA, IgM, IgE, complement C3, complement C4, D-dimer and 24 h urine protein were analyzed between groups. Results Leukocyte count, lymphocyte percentage, platelet count, CD3+CD8+, CD-CD19+, IgA, IgE, complement C3, D-dimer and 24 h urine protein in HSP group were significantly higher than those in control group[ (8.7 ±3.5)×10^9/L vs (5.9 ± 1.6)× 10^9/L, (39 ± 16)% vs (35 ± 6)%, (275 ± 79) × 10^9/L vs (219±65) ×10^9/L,(30±6)% vs (24 ±4)%,(19±10)% vs (13 ±3)%,(2.5±1. 1)g/L vs (1.4± 0.4)g/L,(170 ± 168) kIU/L vs (46 ± 34) kIU/L, (1. 30 ± 0. 32) g/L vs (1.23 ± 0. 24) g/L, ( 1 840 ± 1 720 ) μg/L vs ( 260 ± 120 ) μg/L, 0.10 ( 0.04,0.48 ) g vs 0.06 ( 0.04,0.09 ) g ] ( P 〈 0.05 ) ; CD3+, CD3+ CD4+, CD16+ CD56+ and IgG in HSP group were significantly lower than those in control group[ (67 ± 9)% vs (81 ± 5 )%, (32.1±1.7)% vs (52.6±3.3)%,(9±3)% vs (15±5)%,(8.0±3.0)g/L vs (8.7±

关 键 词:紫癜  过敏性 紫癜性肾炎 标志物 

分 类 号:R554.6[医药卫生—血液循环系统疾病]

 

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