ToxoDB#17型弓形虫卵囊对昆明小鼠致病性的研究  

Virulence and pathogenicity of Toxoplasma gondii ToxoDB#17 strain oocysts in Kunming mice

作　　者：王凯[1] 冯永杰[1] 付晓莹[1] 陆瑶瑶[1] 梁宏德[1] 杨玉荣[1] 

机构地区：[1]河南农业大学牧医工程学院,郑州450002

出　　处：《中国人兽共患病学报》2017年第1期49-52,共4页Chinese Journal of Zoonoses

基　　金：国家自然科学基金(No.30800812);河南省自然科学基金资助(No.162300410138)~~

摘　　要：目的为探究ToxoDB#17型弓形虫对昆明小鼠的致病特点。方法分别选择<1个,1个,101个,102个,103个,104个,105个,106个ToxoDB#17型弓形虫卵囊灌胃小鼠,观察小鼠的临床症状,采用改良凝集试验、HE和IHC方法研究弓形虫的感染率,小鼠生存率及其大脑内的包囊数量。结果≥104个卵囊组小鼠在攻虫后出现一过性的精神沉郁,弓背,被毛逆立现象,其余组精神状态正常;≥102个卵囊可引起小鼠100%感染,感染小鼠的成囊率为2.38%(1/42),感染小鼠生存率为85.71%(36/42),存活时间≥360DPI。结论 ToxoDB#17型弓形虫对昆明小鼠的致病力弱,包囊形成率低。To investigate the pathogenic characteristic of ToxoDB#17 Toxoplasma gondii in Kunming mice, the mice were fed with 〈1, 1, 10^1, 10^2, 10^3, 10^4, 10^5, 106 oocysts, and the clinical symptoms of mice were observed. MAT, HE and IHC were used to research the infection rate of T. gondii, survival rate of mice and the number of cysts in brain. The results showed that the mice fed with ≥10^4 oocysts appeared transient depression, mice were arched, messy hair after infection. The mice fed with ≥10^2 oocysts were all infected, T. gondii cysts formation rate was 2.38% （1/42）, the survival rate of infected mice was 85.71% （36/42）, the survival time was greater than 360 days. In conclusion, the pathogenicity of ToxoDB#17 T. gondii is weak, and cysts formation rate is also low.

关 键 词：ToxoDB#17型弓形虫 昆明小鼠 包囊 致病性 

分 类 号：R382.5[医药卫生—医学寄生虫学]