一起不明原因群体性肺部感染的病原体分离及鉴定  

Pathogen isolation and identification of an outbreak of infection in lung with unknown causes

作　　者：石一[1] 张义[1] 张铮[1] 史伟[1] 马国柱[1] 刘长宏[1] 雷金娥[2] 马琳[1] 曹磊[1] 张志成[1] 刘峰[1] 刘东立[1] 

机构地区：[1]陕西省疾病预防控制中心,西安710054 [2]西安交通大学第一附属医院,西安710061

出　　处：《中国人兽共患病学报》2017年第1期57-60,共4页Chinese Journal of Zoonoses

基　　金：陕西省卫生和计划生育委员会科研项目(D11)资助~~

摘　　要：目的调查了解一起不明原因群体性肺部感染的病原菌。方法通过流行病学分析,运用荧光定量PCR、ELISA、胶体金、VITEK2生化鉴定以及MALDI-TOF-MS飞行时间质谱技术对可疑菌进行鉴定,并将分离到的可疑菌与病例血清进行凝集试验。结果从环境标本中分离到2株浅白隐球菌,并与病例血清发生特异性凝集。结论此次肺部感染可能与浅白色隐球菌感染有关。We investigated the pathogen of an outbreak of lung infection with unknown causes. By epidemiological analysis, we used real-time PCR, ELISA, gold dipstick, VITEK2 and MALDI-TOF-MS to identify suspicious bacteria. We made use of serum plate agglutination test to confirm the suspicious bacteria and the patient serum. We isolated 2 strains of Cryptococcus albidus from environmental samples. There has been specific agglutination between suspicious bacteria and patient serum. This pneumonia may be related to the infection of Ccryptococcus albidus.

关 键 词：肺炎 浅白隐球菌 PCR MALDI-TOF-MS 血清凝集 

分 类 号：R379.5[医药卫生—病原生物学]