草鱼D-loop多态性与幼苗生长性状的关联分析  

Associations between D-loop polymorphisms and offspring growth traits in Ctenopharyngodon idella

作　　者：傅建军[1,2] 张猛[1] 沈玉帮[1] 陈勇 李家乐[1] FU Jian -jun ZHANG Meng SHEN Yu -bang CHEN Yong LI Jia -le(Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 201306, China Key Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081 Jiangsu, China Aquatic Engineering Technology Research Center, Tongwei Group Co. , Ltd. , Chengdu, 610093, China)

机构地区：[1]上海海洋大学,省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [2]中国水产科学研究院淡水渔业研究中心,农业部淡水渔业与种质资源利用重点实验室,无锡214081 [3]通威股份有限公司水产工程技术研究中心,成都610093

出　　处：《淡水渔业》2017年第1期17-21,共5页Freshwater Fisheries

基　　金：国家科技支撑计划(2012BAD26B02);现代农业产业技术体系建设项目(CARS-46-04);上海市种业发展项目(2012NY10);通威股份有限公司产学研项目(TW2014F003)

摘　　要：为了探索草鱼(Ctenopharyngodon idella)线粒体DNA(mitochondrial DNA,mt DNA)多态性对生长性状的影响,鉴于mt DNA的母性遗传特征,本研究基于2011年繁殖用的20尾母本的D-loop序列信息,与通过亲子鉴定获得的853尾40日龄子代的体长、体质量进行关联分析。结果显示,草鱼6种D-loop单倍型对生长性状表型差异具有极显著影响(P<0.01);其中,单倍型为Hap16的子代的体长最大,并显著大于单倍型为Hap4的子代的体长(P<0.05);单倍型为Hap18和Hap16的子代的体质量较大,依次大于其他单倍型子代的体质量,并显著大于单倍型为Hap4的子代的体质量(P<0.05)。此外,草鱼D-loop序列各变异位点基因型对生长性状的影响水平不同;其中,Site01、Site06和Site07等3个位点对体长的差异存在显著影响(P<0.05),Site06和Site07等2个位点对体质量的差异存在显著影响(P<0.05)。研究表明,草鱼D-loop序列变异对子代生长性状具有显著影响,推测在草鱼生长性状改良的选育进程中,可以利用mt DNA多态性信息进行辅助选择。Aimed to analyze the association between mt DNA（ mitochondria DNA） polymorphisms and offspring growth traits in Ctenopharyngodon idella,in view of the maternal inheritance of mt DNA,the haplotypes and variable site genotypes of D- loop sequence in twenty dams（ induced breeding carried out in 2011） were used to evaluate the association with 40pfd（ post- fertilization days） offspring growth traits of 853 individuals under molecular pedigree. There were highly significant differences on offspring growth traits based on different D- loop haplotypes detected in the current study（ P〈0. 01）.The largest of standard length was detected in offspring with Hap16 D- loop haplotype,and it was significantly larger than the offspring with Hap4 D- loop haplotype（ P〈0. 05）; larger of body weight were detected in offspring with Hap18 and Hap16 D- loop haplotypes compared to offspring with other haplotypes,and they were significantly larger than offspring with Hap4 D- loop haplotypes（ P〈0. 05）. Furthermore,different influences for offspring growth traits were detected among different variable sites of D- loop haplotypes. Three variable sites of D- loop haplotypes（ Site01,Site06 and Site07）were showed with significant effects for offspring standard length（ P〈0. 05）,and two sites（ Site06 and Site07） wereshowed with significant effects for offspring body weight（ P〈0. 05）. In conclusion,there were significant associations between the D- loop polymorphisms and offspring growth traits in C. idella,therefore,the dam mt DNA polymorphism should be an assistant molecular tool for practical breeding program in C. idella.

关 键 词：草鱼(Ctenopharyngodon idella) D-loop序列 多态性 生长性状 关联性 

分 类 号：S917.4[农业科学—水产科学]