氧化酶法和钒酸盐法测定血清胆红素的方法比较  

Developed for the Determination of Serum Bilirubin between Oxidase Method and Vanadate Oxidation Method

作　　者：郝树彬[1] 孙洪厚 孙秀会 刘兴翠 王志全[2] 孙爱国 

机构地区：[1]山东省医疗器械产品质量检验中心/山东省医疗器械生物学评价重点实验室,济南250101 [2]莘县中日友好医院,山东聊城252400 [3]潍坊泽城生物技术有限公司,山东潍坊261100 [4]山东省恒信检测技术开发中心,济南250101 [5]青州荣军医院,山东潍坊262500

出　　处：《现代检验医学杂志》2017年第1期113-116,120,共5页Journal of Modern Laboratory Medicine

摘　　要：目的通过对测定结果进行比对,研究胆红素钒酸盐氧化法和胆红素氧化酶法的反应方法原理,探讨两种方法之间异同点及结果是否具有可比性。方法以两种方法分别检测310例血清,计算回归方程,评估两种方法的相关性。对异常样本进一步实验以研究方法之间的异同。结果总胆红素测定结果的回归方程为Y=1.065 1X+1.197 2,相关系数r=0.997 0。成年人及>30天儿童直接胆红素测定结果回归方程为Y=0.945 9X+0.599 5,相关系数r=0.994 4;新生儿测定结果的回归方程为Y=0.410 4X+2.756 3,相关系数r=0.883 5;结果异常新生儿血清梯度稀释后钒酸盐氧化法结果不可接受。结论总胆红素和成人及>30天儿童的直接胆红素两种方法测定结果的相关性在可接受范围内;新生儿直接胆红素测定两种方法的相关性不可接受;钒酸盐氧化法不可用于新生儿直接胆红素测定。Objective To study reaction principle of bilirubin vanadate oxidation method and bilirubin oxidase method through comparison of the determination results,and discuss similarities and difference between the two methods . Methods 310 ca- ses were measured and analyzed with each method. Abnormal sampleswere further investigated. Results Fortotal bilirubin, the regression equation obtained was Y= 1. 065 1Xq-1. 197 2, the correlation coefficient r= 0. 997 0. For direct bilirubin of a- dults and children greater than 30 days,the regression equation was Y=0. 945 9X＋0. 599 5 and the correlation coefficient r =0. 994 4. For neonatal direct bilirubin,the regression equation was Y=0. 410 4X＋2. 756 3 and the correlation coefficient r = 0. 883 5. The results from vanadate oxidation method were unacceptable for abnormal neonatal serum measurement after serial dilution. Conclusion The overall conclusions were that for the measurement of total bilirubin,and direct bilirubin for adults and children older than 30 days. The correlation between these two methods is in an acceptable range, for measure- ment of neonatal direct bilirubin, the correlation between thesetwo methods was not acceptable. It is not recommended to measure neonatal direct bilirubin by vanadate oxidation method.

关 键 词：总胆红素 直接胆红素 胆红素氧化酶法 钒酸盐氧化法 新生儿直接胆红素 

分 类 号：R446.112[医药卫生—诊断学]