Ghrelin对绵羊早期体外胚胎发育调节机制的初步研究  

Regulation of early ovine embryos development in vitro by Ghrelin

作　　者：杨燕燕[1,2] 达来 张冠华[1] 彭灿泉 郭延华[3] 曹贵方[1] YANG Yanyan DA Lai ZHANG Guanhua PENG Canquan GUO Yanhua CAO Guifang(College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia 010018, China Institute of Animal Husbandry, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot, Inner Mongolia 010031, China Institute of Animal Husbandry and Veterinary Medicine, Xinj iang Academy of Agricultural and Reclamation Science, Shihezi, Xinjiang 832000, China)

机构地区：[1]内蒙古农业大学兽医学院,内蒙古呼和浩特010018 [2]内蒙古自治区农牧业科学院畜牧研究所,内蒙古呼和浩特010031 [3]新疆农垦科学院畜牧兽医研究所,新疆石河子832000

出　　处：《西北农林科技大学学报（自然科学版）》2017年第2期1-10,共10页Journal of Northwest A&F University(Natural Science Edition)

基　　金：国家自然科学基金项目(30860201)

摘　　要：【目的】探讨Ghrelin对绵羊早期胚胎发育的调节机制。【方法】将绵羊体外受精早期胚胎在Ghrelin组(300,1 000,10 000ng/mL)、Ghrelin受体拮抗剂D-Lys3-GHRP-6组(10,200,400ng/mL)、D-Lys3-GHRP-6+Ghrelin组(D-Lys3-GHRP-6 400ng/mL,Ghrelin 300,1 000和2 000ng/mL)、空白对照(不加D-Lys3-GHRP-6和Ghrelin)4个组别的胚胎发育液中进行发育培养,采用荧光定量PCR技术分别对各处理不同发育阶段(2-细胞期、4-细胞期、8-细胞期和16-细胞期)绵羊早期胚胎中ERK1、ERK2、P90rsk、Bcl-2、Bax mRNA的表达情况进行检测。【结果】与空白对照组相比,在添加Ghrelin组ERK1、ERK2、P90rsk、Bcl-2基因mRNA的表达表现为显著性上调(P<0.05),且这种上调作用可被D-Lys3-GHRP-6所拮抗;在添加Ghrelin组Bax基因mRNA的表达无显著性变化,但在D-Lys3-GHRP-6组Bax基因mRNA的表达表现为显著性上调(P<0.05)。【结论】Ghrelin可级联激活MAPK信号通路,抑制促凋亡基因Bax的表达、促进抗凋亡基因Bcl-2的表达,进而促进绵羊早期胚胎的发育。【Objective】The purpose of this study was to investigate the regulation mechanism of ghrelin during the development of early ovine embryos.【Method】Early ovine embryos were cultured in four groups with different media：Ghrelin（300,1 000,and 10 000ng/mL）,D-Lys3-GHRP-6（Ghrelin receptor antagonist;10,200,and 400ng/mL）,D-Lys3-GHRP-6plus Ghrelin（D-Lys3-GHRP-6：400ng/mL,Ghrelin300,1 000,and 2 000ng/mL）,and the control group（D-Lys3-GHRP-6：0ng/mL and Ghrelin 0ng/mL）.The mRNA expression levels of ERK1,ERK2,P90 rsk,Bcl-2,and Bax were analyzed during the same stages（2-cell,4-cell,8-cell,and 16-cell）.【Result】Compared with the control group,mRNA expressions of ERK1,ERK2,P90 rsk,and Bcl-2were upregulated significantly（P〈0.05）,and the upregulated functioncould be blocked by D-Lys3-GHRP-6.Bax mRNA expression was insignificantly different in Ghrelin groups but it was upregulated significantly in D-Lys3-GHRP-6group（P〈0.05）.【Conclusion】Ghrelin promoted the development of early ovine embryos by activing the MAPK pathway,inhibiting the expression of Baxgene and promoting the expression of Bcl-2gene.

关 键 词：GHRELIN 绵羊 早期胚胎 MAPK 凋亡 

分 类 号：S851.347.1[农业科学—预防兽医学]