激光捕获显微切割技术在强奸案中的检验研究  

Applying Laser Capture Microdissection to Separate the Shapedifferent Cells for Testing the Mixed Samples in Rape Cases

作　　者：俞卫东[1] 连昌舟[1] 孙大鹏[1] YU Weidong LIAN Changzhou SUN Dapeng(Institute of Forensic Science, Nanjing Public Security Bureau, Nanjing 210012, China)

机构地区：[1]南京市公安局刑事侦查局,南京210012

出　　处：《刑事技术》2017年第1期25-27,共3页Forensic Science and Technology

基　　金：南京市公安局科研项目(No.KY2014009)

摘　　要：目的研究建立一套以激光捕获显微切割技术(LCM)分离强奸案混合样本的检验方法。方法通过不同的样本制备、染色并比较DNA分型结果,确立了以去离子水处理样本,苏木素或甲基紫染色制备样本并经细胞涂片、切割收集进行DNA检测与分型的方法。结果建立的方法能从15个上皮细胞、50个精子细胞中稳定检出正确且完整的STR分型。结论本实验方法可用于强奸案中混合斑的检验。Objective To establish a method of separating different cells in mixtures from rape cases with laser capture microdissection（LCM） technique. Methods Sample preparation was tried with different methods, and so was the cell-staining choices. Therefore, through the sample to be treated with pure water plus hematoxylin or methyl violet to stain the cells that were subjected to painting and dissecting for collection by LCM, DNA has been harvested to result in its tested genotypes showing correct. Results The full and correct STR profiles can be stably obtained from no less than 15 somatic or 50 sperm cells. Conclusion The method established in this paper can be used for testing the mixtures of rape cases.

关 键 词：法医物证 激光捕获显微切割技术 混合样本 STR分型 

分 类 号：DF795.2[医药卫生—法医学]