高温刺激导致虾夷扇贝死亡因素的探究  

Pilot study on reasons for yesso scallop's(Patinopecten yessoensis) survival after heat shock

作　　者：刘超[1,2,3,4] 吴富村[1,3,4] 林思恒[1,2,3,4] 阙华勇[1,3,4] 张国范[1,3,4] LIU Chao WU Fu-cun LIN Si-heng QUE Hua-yong ZHANG Guo-fan(Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China University of Chinese Academy of Sciences, B eijing 100049, China Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China National ＆ Local Joint Engineering Laboratory of Ecological Mariculture, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China)

机构地区：[1]中国科学院实验海洋生物学重点实验室,山东青岛266071 [2]中国科学院大学,北京100049 [3]青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室,山东青岛266071 [4]中国科学院海洋研究所海洋生态养殖技术国家地方联合工程实验室,山东青岛266071

出　　处：《海洋科学》2016年第11期91-98,共8页Marine Sciences

基　　金：现代农业产业技术体系建设专项(CARS-48);山东省泰山学者攀登计划项目;中国科学院科技服务网络计划项目(KFJ-EW-STS-060)~~

摘　　要：本文探究了高温刺激导致一龄和二龄虾夷扇贝死亡的原因。在高温刺激以及恢复过程中,两种贝龄的扇贝存活率存在显著差异(P<0.05),同时两者的谷丙转氨酶(glutamic-pyruvic transaminase,GPT)活性、p53蛋白含量、总抗氧化能力(total antioxidant capacity,TAOC)以及HSP70(heat shock protein 70)含量在高温刺激以及恢复过程中的变化均存在显著差异。而且这些酶的活性(TAOC活性除外)以及蛋白质含量(p53以及HSP70)均受到贝龄、处理时间以及两者综合作用的显著影响。经过Cox模型分析可知,虾夷扇贝受到高温刺激后的存活受到贝龄、GPT活性、TAOC活性、p53含量以及HSP70含量的显著影响。此外,高温刺激会影响扇贝的心跳频率以及心输出量,从而影响心脏对机体的供氧供血能力,影响扇贝对高温的适应能力。In this study, one- and two-year old yesso scallops were used to explore the influence of heat shock on scallop survival, physiological activity involved in immunity and metabolism, and heart rate. The differences in the above factors between the two types of scallop were also determined. During heat shock and recovery, the one-year old scallops＇ survival rate was significantly higher than that of the two-year scallops. In addition, the GPT activity, p53, and HSP70 were significantly different between the two types of scallops. The multiple factor variance analysis demonstrated that the enzyme activity （TAOC） and protein concentration （p53 and HSP70） were influenced by age, processing time, and the interaction between them significantly. The Cox model showed the significant contri- bution of age, GPT, TAOC, p53, and HSP70 to scallops＇ survival. In addition, heat shock can affect heart rate.

关 键 词：虾夷扇贝(Patinopecten yessoensis) 存活曲线 谷丙转氨酶 总抗氧化能力 HSP70 P53 心率 

分 类 号：S917.4[农业科学—水产科学]