柴胡属5种易混药材的鉴别研究  

Identification on Five Kinds of Easily Confused Bupleurum Medicinal Materials

作　　者：丁锤[1,2] 徐莹[1,2] 马孝熙[1,2] 王志鹏[1,2] 毛雯雯[1,2] 张兰兰[1,2] 

机构地区：[1]天士力控股集团有限公司,天津300410 [2]安国数字本草检验中心有限公司,河北保定071200

出　　处：《中药材》2016年第9期1975-1981,共7页Journal of Chinese Medicinal Materials

摘　　要：目的:对柴胡属5种药材北柴胡、南柴胡、竹叶柴胡、藏柴胡和锥叶柴胡进行性状、薄层色谱及分子鉴定研究。方法:参考2015年版中国药典四部相关方法,对药材进行了性状观察和描述;以柴胡皂苷d和柴胡对照药材为指标性成分和标准物质,建立了柴胡属5种易混药材的薄层色谱鉴别方法;采用DNA条形码鉴别方法对5种易混药材进行了鉴别研究。结果:5种药材从性状特征上能够准确予以区分;薄层色谱结果显示,锥叶柴胡和其他几种柴胡区别较大,能够快速鉴别,竹叶柴胡及藏柴胡与正品柴胡较为一致;分子鉴定结果表明ITS2序列可用于5种柴胡药材的物种鉴定。结论:该研究建立的性状及薄层色谱方法在鉴别这5种药材上具有简单、准确的特点,DNA条形码技术是传统鉴别方法的有效补充,具有很大的应用前景,该研究也为竹叶柴胡和藏柴胡进一步的药效学研究提供了科学依据。Objective： Macroscopic identification,thin layer chromatography（ TLC） identification and molecular identification based on the Chinese Pharmacopoeia were established to identify five kinds of Bupleurum medicinal materials such as Bupleurum chinense,Bupleurum scorzonerifolium,Bupleurum marginatum,Bupleurum marginatum var. stenophyllum and Bupleurum bicaule,which were easily confused. Methods： According to the Chinese Pharmacopoeia,macroscopic characters of these five kinds of Bupleurum medicinal materials were observed and described; TLC identification method with saikosaponin d and control medicinal material of Bupleuri Radix as reference substances was established. Furthermore,DNA barcode was applied in identification of these confused drugs. Results： Five Bupleurum medicinal materials could be easily distinguished by their morphological characters. Moreover,TLC of Bupleurum chinense,Bupleurum scorzonerifolium,Bupleurum marginatum and Bupleurum marginatum var. stenophyllum were extremely similar while being different from that of Bupleurum bicaule. The ITS2 sequences could be used to identify the five Bupleurum medicinal materials. Conclusion：The established macroscopic identification and TLC for distinguishing of the five kinds of Bupleurum medicinal materials are simple and accurate while DNA barcode technology being effective and extensive. Besides,this study provides scientific basis for the further pharmacodynamic studies on Bupleurum marginatum and Bupleurum marginatum var. stenophyllum.

关 键 词：柴胡属 性状鉴别 薄层鉴别 DNA条形码 柴胡皂苷D 

分 类 号：R282.5[医药卫生—中药学]