超临界流体萃取红豆杉枝叶紫杉烷类萃余物中紫杉黄酮及单体组分的工艺优化  

Optimization of Supercritical Fluid Extraction of Total Flavonoids and Monomer Compositions from Taxus Remainder Extracts Free of Taxoids

作　　者：李兆慧[1] 严柳叶 喻晓雁[1] 阮晓[2] 刘本[2] 王强[2] 

机构地区：[1]浙江医药高等专科学校中药学院,浙江宁波315100 [2]浙江大学宁波理工学院生化分院,浙江宁波315100

出　　处：《中药材》2016年第9期2062-2067,共6页Journal of Chinese Medicinal Materials

基　　金：浙江省中医药科技计划项目(2013ZA120);浙江省教育厅科研资助项目(Y201330188)

摘　　要：目的:采用响应面法优化红豆杉枝叶紫杉烷类萃余物(TREFT)中黄酮类物质的超临界流体萃取工艺。方法:根据Central Composite Design设计原理,采用四因素五水平的响应面分析法,分别考察萃取时间、萃取温度、萃取压力和夹带剂乙醇浓度对紫杉黄酮及单体组分阿曼托黄酮、槲皮素、银杏素提取率的影响。结果:优化工艺条件为:萃取时间2.2 h,萃取温度46.4℃,萃取压力22.6 MPa,夹带剂乙醇浓度80.7%,紫杉黄酮及单体组分提取率的综合评分为98.75。结论:优选的超临界流体萃取紫杉黄酮及单体组分工艺稳定可行,可为TREFT资源化利用提供技术支持。Objective： To optimize the supercritical fluid extraction technology of flavonoids from taxus remainder extracts free of taxoids（ TREFT） by response surface methodology（ RSM）. Methods： By using a central composite design（ CCD） in four factors and five levels to optimize the extraction parameters. The effects of extraction time,temperature,pressure and different concentration of ethanol and their interaction on the extraction rate of total flavonoids,amentoflavone,quercetin and ginkgetin which extracted from TREFT by supercritical fluid CO2 were investigated. Results： Under optimal conditions of extraction time for 2. 2 h,and the extraction temperature was 46. 4 ℃,the extraction pressure was 22. 6 MPa,and 80. 7% ethanol,the index experimental of total flavonoids and monomer compositions was 98. 75. Conclusion： The process of flavonoids extraction from TREFT is stable and feasible,which can provide technical support for the resource utilization of TREFT.

关 键 词：红豆杉枝叶紫杉烷类萃余物 紫杉黄酮 超临界CO2萃取 响应面分析法 

分 类 号：R283.6[医药卫生—中药学]