竹节参总皂苷对自然衰老大鼠小肠上皮细胞紧密连接蛋白的调节作用及其机制研究  

Effects of the Saponins from Panax japonicus on Intestinal Epithelial Tight Junction Proteins in Aging Rats

作　　者：王海英[1] 顿耀艳[1] 袁丁[2] 刘敏[1] 郭煜晖[1] 张长城[1] 

机构地区：[1]三峡大学医学院,湖北宜昌443002 [2]三峡大学仁和医院,湖北宜昌443002

出　　处：《中药材》2016年第9期2091-2096,共6页Journal of Chinese Medicinal Materials

基　　金：三峡大学人才科研启动基金(KJ2012B060;KJ2013B011);宜昌市科技局项目(A15301-36);国家自然科学基金(81503423;81403307)

摘　　要：目的:研究竹节参总皂苷对衰老大鼠肠道上皮细胞紧密连接蛋白表达的调节作用及其可能机制。方法:SPF级SD大鼠分为成年组(6月龄)、衰老模型组(24月龄)及竹节参总皂苷高、中、低剂量组5组。免疫组织化学方法检测各组大鼠回肠上皮细胞间紧密连接蛋白Occludin、ZO-1,抗氧化应激相关蛋白Nrf2、HO-1、NQO-1,线粒体相关蛋白Sirt1、PGC-1α,及p-AMPK蛋白的表达情况。结果:与成年组大鼠比较,自然衰老大鼠Occludin、ZO-1、Nrf2、HO-1、NQO-1、Sirt1、PGC-1α、p-AMPK蛋白表达均显著降低(P<0.01)。与自然衰老大鼠比较,竹节参总皂苷各组大鼠Occludin、ZO-1、Nrf2、HO-1、NQO-1、Sirt1、PGC-1α、p-AMPK表达显著升高(P<0.05或P<0.01)。结论:自然衰老大鼠细胞间紧密连接蛋白表达减少引起的回肠黏膜屏障功能障碍可能与线粒体氧化应激密切相关,而竹节参总皂苷可能通过调节衰老大鼠线粒体氧化应激的水平,从而上调肠上皮细胞间紧密连接蛋白的表达,改善衰老大鼠肠道黏膜屏障功能障碍。Objective： To investigate the moderating effects of total saponins of Panax japonicus on intestinal epithelial tight junction proteins in aging rats,and to explore the potential mechanism. Methods： SD rats were divided into adult group（ 6 months）,old model group（ 24 months）,and different doses（ 10,30 and 60 mg / kg） of total saponins of Panax japonicus treatment groups. Levels of tight junction proteins（ Occludin and ZO-1）,anti-oxidative pathway proteins（ Nrf2、HO-1 and NQO-1）,mitochondrial biogenesis related proteins（ Sirt1 and PGC-1α） and p-AMPK in the ileum were determined by immunohistochemistry staining. Results： Compared with adult group,the expressions of Occludin,ZO-1,Nrf2,HO-1,NQO-1,Sirt1 and PGC-1α of aging rats were obviously decreased（ P〈0. 01）,and p-AMPK was inhibited in the ileum of aging rats. Compared with aging model rats,total saponins of Panax japonicus increased the expressions of Occludin,ZO-1,Nrf2,HO-1,NQO-1,Sirt1 and PGC-1α（ P〈0. 05 or P〈0. 01）,and activated p-AMPK in the ileum of aging rats. Conclusion： The decreased level of intestinal tight junction proteins in the ileum of aging rats may be related to oxidative stress. Total saponins of Panax japonicus can up-regulate the level of intestinal tight junction proteins to improve the intestinal mucosal barrier dysfunction in the ileum of aging rat via reducing the levels of oxidative stress.

关 键 词：竹节参总皂苷 紧密连接蛋白 衰老 

分 类 号：R285.5[医药卫生—中药学]