^(137)Cs-γ辐照及5-氮杂胞苷处理毛竹种子对其实生苗甲基化水平的影响  

Effects of Treating Bamboo Seeds With ^(137)Cs-γ Ray and 5-azacytidine on Methylation Level of Bamboo Seedling

作　　者：陈昂[1] 周明兵[1] 汤定钦[1] 

机构地区：[1]浙江农林大学林业与生物技术学院/亚热带森林培育国家重点实验室培育基地,浙江临安311300

出　　处：《核农学报》2017年第2期218-224,共7页Journal of Nuclear Agricultural Sciences

基　　金：浙江自然科学基金杰出青年项目(LR12C16001);国家自然科学基金项目(31270645;31470615)

摘　　要：为了研究药物和辐照对毛竹甲基化水平的影响,采用0、25、50、100、250μmol·L-1的5-氮杂胞苷和0、10、20、30、40、60、90、120 Gy的^(137)Cs-γ射线分别处理毛竹种子,并利用高效液相色谱技术,采用外标法测定实生苗叶片的甲基化水平。结果表明,5-氮杂胞苷处理毛竹种子后,甲基化水平下降,其中250μmol·L-1处理后甲基化水平为20.8%,下降最显著;低剂量的辐照处理对甲基化的影响不明显,而在高剂量的γ射线胁迫下,DNA甲基化程度下降至26.0%,达到极显著水平。本研究揭示了辐照与基因组甲基化水平间的相关性,为毛竹辐照诱变和甲基化抑制育种提供了科学依据。In order to study the effects of drugs and radiation on bamboo DNA methylation level, moso bamboo （Phyllostachys edulis） seeds were soaked in 5 - azaC solution with different concentration gradient （0, 25, 50, 100, 250μmol·L-1） and radiated by 137Cs -3, ray with different dose gradient （0, 10, 20, 30, 40, 60, 90, 120 Gy） before seedling. The methylation levels of seedling leaves were measured by high performance liquid chromatography with external standard method. The results showed that after treatment with different concentrations of 5 - azaC, DNA methylation level of bamboo was decreased. The DNA methylation level of cell was lowest （20.8%） under the concentration of 250μmol·L-5 - azaC. Low dose of radiation treatment had few effects on methylation. After being treated by high doses γ ray, DNA methylation level decreased to 26.0% , reaching a significant level. This study showed the feasibility of manual decrease of cell DNA methylation by directly methylation inhibitor treatment on bamboo seeds and revealed the correlation between dose of γ- ray irradiation treatment and genomic methylation, provided scientific basis of application drugs and radiation on moso bamboo breeding.

关 键 词：毛竹实生苗 5-氮杂胞苷 辐照诱变 DNA甲基化 高效液相色谱 

分 类 号：S795.7[农业科学—林木遗传育种]