库姆塔格沙漠北界阿奇克谷地土壤可培养细菌多样性  

The Diversity of Cultivable Bacteria of Soil in Aqik Valley,the North Boundary of Kumtag Desert

作　　者：许璐[1] 曹晓璐[1] 王士燕[1] 孙磊[2] 李潞滨[1] 

机构地区：[1]中国林业科学研究院林业研究所,北京100091 [2]河北大学生命科学学院,河北保定071002

出　　处：《核农学报》2017年第2期342-349,共8页Journal of Nuclear Agricultural Sciences

基　　金：国家林业局948项目(2011-4-71)

摘　　要：为明确库姆塔格沙漠北界阿奇克谷地的土壤细菌多样性,探索该地区土壤微生物与植物群落的相互作用关系,采用NA、R2A培养基分离培养土壤细菌,通过16S rDNA序列系统发育分析鉴定菌株。结果表明,R2A平板菌落数量和种类较多;发现13株潜在新种菌株,已知菌种分属3大类群、4属、20种,厚壁菌门(Firmicutes)为优势类群,芽胞杆菌属(Bacillus)为优势属,枯草芽胞杆菌(Bacillus subtilis subsp.inaquosorum)为优势菌种。与其他类似环境相比,阿奇克谷地土壤中可培养细菌生物量偏小,可培养的细菌多为抗性、耐性强的极端微生物。该地区特有的微生物资源、功能微生物和新菌资源丰富,为防沙治沙、微生物菌剂开发等研究提供物质基础。本研究结果对库姆塔格沙漠的自然生态保护以及极端环境微生物资源的应用具有重要意义。In order to research the bacterial diversity of soil in Aqik Valley and to explore the interaction between the soil microbe and vegetational community, the NA and R：A solid media were applied to isolate and culture the bacteria from the soil samples, and 16S rDNA sequence analysis were used to dentify the isolates. The results showed that the most quantity and species of bacterial colonies were found on R2A media, and 13 isolates were potential new bacteria and the other isolates were classified into 3 taxa, 4 genus and 20 species. The dominant taxon was Firmicutes, the dominant genus was Bacillus, and the dominant species was Bacillus subtilis subsp, inaquosorum. Compared with other similar environments, the biomass of cultivable bacteria of soil in Aqik Valley was low, and the majority of cultivable bacteria were extremophiles with strong resistance and salt tolerance. There are abundant microbial resources, functional microorganisms and new bacteria resources in this area, providing good material basis for the desert control, microbial agent development and related research. This study have important implications for natural ecosystem protection and extremophile species resource utilization.

关 键 词：阿奇克谷地 细菌 16S RDNA 植被群落组成 

分 类 号：S154.3[农业科学—土壤学]