慢型克山病患者血清中αB晶体蛋白表达及意义的研究  

Serum level of HSPB5 and its significance with chronic Keshan disease

作　　者：陈向丽[1] 王铜[1] 冯红旗[1] 侯杰[1] 甄荣霞 徐春艳[1] 王哲[1] CHEN Xiang-li WANG Tong FENG Hong-qi HOU Jie ZHEN Rong-xia XU Chun-yan WANG Zhe(Institut of Keshan Disease, Chinese Center for Endemic Disease Control, Harbin Medical University, Harbin 150081, China)

机构地区：[1]哈尔滨医科大学中国疾病预防控制中心地方病控制中心克山病防治研究所,黑龙江哈尔滨150081

出　　处：《中国地方病防治》2016年第10期1081-1083,共3页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然基金面上项目(81372938;81172607)

摘　　要：目的了解慢型克山病患者血清中αB晶体蛋白的表达情况,探索其是否可以做为克山病生物标志物的一种。方法选择慢型克山病患者和对照各40人。对其血清采用酶联免疫吸附法检测αB晶体蛋白(heat shock protein B5,HSPB5)、N末端B型利钠肽原(NT-pro BNP)、骨桥蛋白(osteopontin,OPN)的表达量,丙二醛(malondialdehyde,MDA)采用比色法测定。结果αB晶体蛋白在病例组中的表达水平(368.51±231.61)低于对照组(486.06±289.08);NT-pro BNP在病例组的表达水平(11 570.61±3 969.37)高于对照组(9 403.79±5 012.81);MDA表达量病例组(5.11±3.49)高于对照组(3.43±2.80);OPN表达量在病例组(37.53±23.10)和对照组(36.59±34.99)间无差异。结论病例组αB晶体蛋白的表达量低于对照组;αB晶体蛋白有可能是克山病心肌损伤的一种心脏标志物。Objective To investigate the expression of αB-crystallin with chronic Keshan disease,to explore whether it can be used as a Keshan disease biomarker.Methods 40 patients with chronic Keshan disease and controls were selected respectively.Serum was collected from the subjects. NT-pro BNP,αB-crystallin and OPN was detected by enzyme-linked immunosorbent assay. MDA was measured by colorimetry.Result The expression level of αB-crystallin in the case group was lower than that in the control group. The expression of NT-pro BNP and MDA was higher than that of the control group. The expression of OPN was similar between the two groups. Conclusion The expression of αB crystallin was lower in the case group than in the control group; αB-crystallin may be a cardiac marker of Keshan disease myocardial injury.

关 键 词：αB晶体蛋白 慢型克山病 心肌损伤 标志物 血清 

分 类 号：R542.3[医药卫生—心血管疾病]