抑郁大鼠模型血浆中文拉法辛和O-去甲基文拉法辛的测定  

Determination of Venlafaxine and O-Desmethylvanlafaxine in Plasma of Depressive Rats

作　　者：胡礼军[1] 江晓佳[2] 卢浩扬[3] 王占璋[3] 倪晓佳[3] 尚德为[3] 胡晋卿[3] 林朝仙[1] 温预关[3] HU Lijun JIANG Xiaojia LU Haoyang WANG Zhanzhang NI Xiaojia SHANG Dewei HU Jinqing LIN Chaoxian WEN Yuguan(The First Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, China The Affiliated Brain Hospital of Guangzhou Medical University（ Guangzhou Huiai Hospital）, Guangzhou , Guangdong 510370, China Jinan University, Guangzhou , Guangdong 510632, China)

机构地区：[1]汕头大学医学院第一附属医院,广东汕头515041 [2]暨南大学,广东广州510632 [3]广州医科大学附属脑科医院(广州市惠爱医院),广东广州510370

出　　处：《今日药学》2016年第12期848-852,共5页Pharmacy Today

基　　金：广州市科技计划项目(2014J4100135);建设中医药强省立项资助科研课题(20141220);广州市医药卫生科技一般引导项目(20161A011037)

摘　　要：目的建立同时检测抑郁大鼠血浆中文拉法辛及其代谢物O-去甲基文拉法辛的高效液相色谱串联质谱(LC-MS/MS)方法。方法采用甲醇直接沉淀法处理大鼠血浆样品。色谱柱为Agilent Eclipse plus-C18(4.6 mm×100 mm,3.5μm),流动相为甲醇-水(60∶40,v/v),流速为0.5 m L·min^(-1)。正离子多反应监测(MRM)扫描分析,离子通道:文拉法辛m/z 278.15→58.20,O-去甲基文拉法辛m/z 264.10→58.10。结果文拉法辛和O-去甲基文拉法辛的线性范围均为4~400 ng·m L^(-1),定量下线均为4ng·m L^(-1),日内、日间精密度RSD均小于11%,相对提取回收率均在90%~105%。结论该方法操作简单,灵敏度高,特异性强,可用于抑郁大鼠血浆内文拉法辛和O-去甲基文拉法辛的含量测定。OBJECTIVE To establish a high performance liquid chromatography-tandem mass spectrometry method for the simultaneous determination of venlafaxine and its metabolite O-desmethylvenlafaxine in plasma of depressive rats. METHODS Plasma samples were prepared by direct methanol precipitation. The chromatographic separation was performed on Agilent Eclipse plus C18（ 4.6 mm× 100 mm, 3.5μm） column with a mobile phase consisted of methanol-water （60：40, v/v ） at a flow rate of 0.5 mL·min^-1. The protonated ions of analytes were detected in positive ionization by multiple reaction monitoring mode （ MRM ）. The mass transition pairs of m/z 278.15→58.20, m/z 264.10→58.10 were used to detected venlafaxine and O-desmethylvenlafaxine. RESULTS The linear concentration range for venlafaxine and O-desmethylvenlafaxine were both 4 - 400 ng· mL^-1. The limit of quantitation for both was 4 ng· mL^-1.Within-batch and between-batch precision RSD were less 11%. The relative extraction recovery was 90%- 105%. CONCLUSION The method has the advantages of simple operation, high sensitivity and specificity, and can be used for the determination studies of venlafaxine and O-desmethylvanlafaxine in depressive rat plasma.

关 键 词：文拉法辛 O-去甲基文拉法辛 高效液相色谱串联质谱法 药代动力学 

分 类 号：R927.2[医药卫生—药学]