基于不同HPLC法测定毛冬青植物不同部位各成分比较  

Comparative studies of chemical component from different parts of Ilex pubescens by different HPLC detector

作　　者：李忠贵 袁海铭 钟小群 李艳 罗强云 王丽静 曾宪仪 

机构地区：[1]江西省医疗器械检测中心,江西南昌330029

出　　处：《南昌大学学报（理科版）》2016年第5期469-473,共5页Journal of Nanchang University(Natural Science)

基　　金：国家十一五支撑计划基金资助项目(2006BAI0601)

摘　　要：采用ELSD-HPLC、UV-HPLC及薄层鉴别法,对毛冬青药材其根、茎、细枝、叶的成分进行对比研究。试验结果显示,毛冬青植物茎、细枝、叶脂溶性成分与根的相似度分别为100%、75%、75%;茎、细枝水溶性成分与根的相似度均为100%。因此认为从化学成分上看,毛冬青药用部位可以从根扩大至根茎或茎。The methods of ELSD-HPLC,UV-HPLC and thin-layer chromatography were used to detect the water-soluble and oil-soluble ingredients from the roots,stems, branches and leaves of the Ilex pubescens. For the oil-solubility ingredients,the similarity between the stems and the roots is 100%,the branches and the roots is 75% ,and the leaves and the roots is 75%. While for the water-soluble ingredients,the similari- ties were all 100%. Therefore,the medicinal part of the Ilex pubescens can be enlarged from root to stem or other overground parts.

关 键 词：毛冬青药材 药物部位 水溶性成分 脂溶性成分 相似性 ELSD-HPLC检测 UV-HPLC 薄层鉴别法 

分 类 号：O656[理学—分析化学]