油樟叶总黄酮的提取及体外抗自由基活性  

Extraction Technology and Anti-radical Activities in vitro of Total Flavonoids from Cinnamomum longepaniculatum Leaves

作　　者：杜永华[1,2] 敖光辉[3] 魏琴[1] 廖小龙[2] 李雪辉[2] 

机构地区：[1]宜宾学院香料植物资源开发与利用四川省高校重点实验室,四川宜宾644000 [2]宜宾学院生命科学与食品工程学院,四川宜宾644000 [3]内江师范学院,四川内江641112

出　　处：《食品研究与开发》2016年第20期32-36,94,共6页Food Research and Development

基　　金：四川省应用基础研究项目(2011JY0050);四川省青年科技创新研究团队培育计划项目(2011JTD0035);四川高校科研创新团队建设计划资助项目(14TD0031)

摘　　要：研究油樟叶总黄酮的提取工艺及其体外抗自由基活性。在单因素试验基础上,采用均匀设计法优化油樟叶总黄酮的提取条件,并通过测定油樟叶总黄酮对DPPH自由基、超氧阴离子自由基和羟自由基的清除率研究其体外抗自由基活性。结果表明,提取油樟叶总黄酮的优化条件为乙醇体积分数60%、液料比34 m L/g、浸泡时间0 min、回流提取时间122 min、提取温度74℃,此条件下总黄酮提取量为42.09 mg/g。油樟叶总黄酮粗品表现出较强的体外抗自由基活性,其对DPPH自由基、超氧阴离子自由基和羟自由基的半数清除浓度分别为2.81、3.35、3.21 mg/m L。The extraction technology and anti-radical activities in vitro of total flavonoids from Cinnamomum longepaniculatum leaves were investigated. The extraction conditions of total flavonoids were optimized using u-niform design on the basis of single factor test, and the anti-radical activities were investigated by measuring the scavenging rate of total flavonoids on DPPH free radical, super oxide anion free radical and hydroxyl radical.＆amp;nbsp;Results showed that the optimum extraction conditions of total flavonoids from C. longepaniculatum leaves were ethanol concentration 60%, ratio of liquid-solid 34 mL/g, soaking time 0 min, extraction time 122 min, extrac-tion temperature 74℃. Under such condition, the extraction rate of total flavonoids was 42.09 mg/g. The crude to-tal flavonoids displayed good anti-radical activities with the half inhibition concentration （IC50） values of 2.81, 3.35,3.21 mg/mL against DPPH free radical, super oxide anion free radical and hydroxyl radical, respectively.

关 键 词：油樟 黄酮 均匀设计 抗自由基 

分 类 号：TQ28[化学工程—有机化工]