枸杞多糖对自发乳腺癌MMTV-PyMT小鼠肿瘤生长和转移的作用  

Inhibitory effect of Lycium barbarum polysaccharide on tumor growth and metastasis in MMTV-PyMT mouse model of breast cancer

作　　者：李媛媛[1] 亓翠玲[2] 周芷晴 杜标炎[1] 李嘉琳[2] 李梦诗[2] 何彦丽[1] LI Yuan-yuan QI Cui-ling ZHOU Zhi-qing DU Biao-yan LI Jia-ling LI Meng-shi HE Yan-li(Guangzhou University of Chinese Medicine, Guangzhou 510006, China Guangdong Pharmaceutical University, Guangzhou 510006, China)

机构地区：[1]广州中医药大学,广州510006 [2]广东药科大学,广州510006

出　　处：《中国实验动物学报》2016年第6期618-621,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：广州中医药大学"薪火计划"资助项目(XH20140102)

摘　　要：目的以MMTV-Py MT小鼠为乳腺癌模型探讨枸杞多糖对乳腺癌生长和转移的作用。方法扩群鉴定转基因自发乳腺癌MMTV-Py MT小鼠,将阳性雌性小鼠随机分为LBP组和对照组,每组8只,小鼠8周龄时给药,LBP组给予LBP(50 mg/kg,腹腔注射)治疗,对照组给予生理盐水,每2 d给药一次测量肿瘤体积一次,4周后脱臼处死,肺Bouin’s溶液固定后观察肺表面转移结节数目,肿瘤组织固定包埋后免疫组化法检测肿瘤细胞增殖及血管密度。结果 MMTV-Py MT阳性小鼠成瘤率100%,与对照组相比,LBP组的肿瘤重量(4.208±0.4463 g)明显地比对照组(6.477±0.3724 g)轻,肿瘤体积也明显比对照组小;LBP组肺表面结节数目(12±1.155个)明显比对照组(20±2.745个)少,免疫组化染色结果显示LBP组肿瘤组织中细胞增殖数目及微血管密度明显比对照组少。结论枸杞多糖可通过抑制MMTV-Py MT小鼠乳腺癌的生长和转移,并抑制肿瘤细胞增殖和血管新生,MMTV-Py MT小鼠可作为乳腺癌肺转移模型运用于抗肿瘤药物研究。Objective To investigate the inhibitory effect of Lycium barbarum polysaccharide（ LBP） on the tumor growth and metastasis in MMTV-PyM T mouse model of breast cancer. Methods The population of MMTV-PyM T transgenic mice was expanded and identified. 8-week old MMTV-PyM T-positive female mice were randomly divided into LBP group and control group,8 mice in each group. The mice of LBP group were given LBP treatment（ 50 mg / kg,i. p.）,and the control group was given normal saline in the same volume,once every 2 days for 4 weeks. The tumor size was measured every two days. The mice were killed at 4 weeks after treatment,the lungs were removed and fixed in Bouin＇ s solution to observe the number of metastatic nodules,and tumor tissues were used for immunohistochemical examination of tumor cell proliferation and vascular density. Results The tumor formation rate was 100% in the MMTV-PyM T-positive mice. The tumor weight of LBP group was 4. 208 ± 0. 4463 g,significantly lower than the 6. 477 g ± 0. 3724 g in the control group（ P〈0. 005）. The number of pulmonary nodules of the LBP group was 12 ± 1. 155,significantly less than that of the control group（ 20 ± 2. 745）（ P〈0. 05）. The immunohistochemical examination using Ki67 and CD31 staining showed that tumor cell proliferation and microvessel density of the LBP group were significantly less than the NS group. Conclusions LBP inhibits breast cancer growth and metastasis through the inhibitory effect on tumor growth and metastasis,inhibition of tumor cell proliferation and angiogenesis in MMTV-PyM T mice. These mice can be used as an ideal model for studies on antitumor drug development for the treatment of breast cancer lung metastasis.

关 键 词：MMTV-PyMT小鼠 枸杞多糖 肿瘤转移 肿瘤生长 

分 类 号：Q95-33[生物学—动物学]