兰州百合鳞茎不定芽诱导和植株再生  

Adventitious Shoots Induction and Plant Regeneration from Bulb Explant of Lilium davidii var. willmottiae

作　　者：胡友军[1] 贾双双[1] 周玉丽[1] 

机构地区：[1]安徽科技学院生命科学学院,安徽凤阳233100

出　　处：《安徽科技学院学报》2016年第6期26-31,共6页Journal of Anhui Science and Technology University

基　　金：安徽科技学院校级重点学科(AKZDXK2015C05)

摘　　要：目的：研究6-苄氨基腺嘌呤（6-BA）和α-萘2酸（NAA）对兰州百合鳞茎的诱导、增殖、生根及试管苗移栽的影响,筛选出不同培养阶段的最佳培养基。方法：选取生长健壮的兰州百合鳞茎,采用MS为基本培养基,添加不同浓度6-BA和NAA,进行不定芽的诱导。以鳞茎诱导的幼苗为外植体,MS添加不同浓度6-BA和NAA进行试管苗增殖培养。将试管苗转入以1/2 MS、MS为基本培养基,添加不同浓度6-BA和NAA组合的生根培养基中进行试管苗的生根培养,当幼根长2-3 cm时,炼苗1-2 d,再移入蛭石和珍珠岩配比为1∶1的营养钵中管理。结果：丛生芽诱导时,6-BA的最适浓度为1.0 mg/L,过高则芽的生长受到抑制;增殖试验中,NAA与6-BA配合使用,可提高芽的增殖率,而且新芽产生时间早,增殖倍数高;1/2 MS和MS培养基中NAA可以提高百合幼苗生根率,6-BA则明显抑制生根量。结论：兰州百合鳞茎片不同部位诱导丛生芽的诱导率顺序为下部〉中部〉上部;经炼苗移栽,再生株成活率可达100%。Objective：To investigate the effects of 6-BA and NAA on the induction,proliferation,rooting and transplanting of test tube seedlings of Lilium davidii var. willmottiae,and to select the best culture medium in different stages. Methods：Select the Lilium davidii var. willmottiae bulb of robust growth,using MS as the basic medium supplemented with different concentrations of 6-BA and NAA for adventitious bud induction. The seedlings were used as explants,MS was added with different concentrations of 6-BA and NAA to test the pro-liferation and culture of test tube seedlings. The plantlets transferred to 1/2 MS and MS as the basic medium supplemented with different concentrations of 6-BA and NAA combination of rooting medium for rooting of plantlets,at the root length of 2 - 3 cm,1 - 2 d seedling,and then into the vermiculite and perlite ratio for the management of nutrition pot in 1 ∶ 1. Results：Bud induction,the optimum concentration of 6-BA for 1 mg/L,high bud growth was inhibited; proliferation test,with the use of NAA and 6-BA,can enhance the bud proliferation rate and sprout time early,high proliferation rate and MS medium; 1/2 MS in NAA can improve the rooting rate of lily seedlings,6-BA inhibited the rooting capacity. Conclusion：In different parts of Lilium davidii var. willmottiae bulb slices induced multiple induction rate order lower middle upper buds; After transplanting,the survival rate of the regenerated plants was 100%.

关 键 词：百合鳞茎 不定芽诱导 植株再生 

分 类 号：Q813.12[生物学—生物工程]