止痒酊质量标准研究  

Research on Quality Standard of Zhiyang Tincture

作　　者：吴磊 陆超 

机构地区：[1]南京中医药大学附属医院药学部,江苏南京210029

出　　处：《安徽科技学院学报》2016年第6期54-57,共4页Journal of Anhui Science and Technology University

基　　金：江苏省中医药管理局项目(JD201512)

摘　　要：目的:建立止痒酊质量标准。方法:首先采用薄层色谱法(TLC),对止痒酊组方中蛇床子和薄荷脑进行定性鉴别,然后采用高效液相色谱法测止痒酊组方中蛇床子素的含量,采用Hedera ODS-3色谱柱(250×4.6 mm,5μm),流动相为甲醇-水(70∶30),流速为1.0 m L/min,检测波长为322 nm。结果:蛇床子素的检测浓度在4.33μg/m L^138.76μg/m L范围内,与峰面积积分值呈良好的线性关系y=86684x+55517(r2=1.0000),平均加样回收率为99.00%,RSD=0.56%(n=6)。结论:所建立的方法准确,专属性强,重现性好,可用于止痒酊的质量控制。Objective：To establish the quality standard for Zhiyang Tincture. Methods：TLC was first used to identify Fruetus Cnidii and Menthol to establish the HPLC method for determining the osthole in Zhiyang Tincture. It was determined on Hedera ODS-3column（250 × 4. 6 mm,5μm）,Mobile phase：methanol-water（70 ∶30）. The flowr ate was at 1. 0 m L/min,and the detive wavelength was at 322 nm. Results：The calibratine curve was linear with a range of 4. 33 - 138. 76 μg for osthole,y = 86684 x ＋ 55517（r^2= 1. 0000）,the average recovery was 99. 00% with RSD% 0. 56%（n = 6）. Conclusion：The methods are accurate with a good reproductivity and can be used as a quantitative analysis for preparation of the osthole in the Zhiyang Tincture.

关 键 词：止痒酊 薄层色谱 高效液相色谱 质量标准 

分 类 号：R917[医药卫生—药物分析学]